Anti-BLOC1S2 Antibody (CAB8007)

Antibody Name:	BLOC1S2 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8007
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 44-142 of human BLOC1S2 (NP_776170.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	NIH/3T3, Mouse liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 44-142 of human BLOC1S2 (NP_776170.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MFSK MATY LTGE LTAT SEDY KLLE NMNK LTSL KYLE MKDI AINI SRNL KDLN QKYA GLQP YLDQ INVI EEQV AALE QAAY KLDA YSKK LEAK YKKL EKR
Gene ID:	282991
Uniprot:	Q6QNY1
Cellular Location:	Cytoplasm, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	11kDa/15kDa
Observed MW:	16kDa

Synonyms:

BLOC1S2, BLOS2, BORCS2, CEAP, CEAP11

Background:

This gene encodes a protein with multiple functions. The encoded protein has been found in association with the centrosome, shown to co-localize with gamma-tubulin, and also found to be one of the proteins in the BLOC-1 complex which functions in the formation of lysosome-related organelles. A pseudogene of this gene is located on the X chromosome. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	BLOC1S2: May play a role in cell proliferation. The BLOC-1 complex is required for normal biogenesis of lysosome-related organelles, such as platelet dense granules and melanosomes. Plays a role in intracellular vesicle trafficking. Belongs to the BLOC1S2 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 10q24.31 Cellular Component: centrosome; recycling endosome; mitochondrion; nucleus; gamma-tubulin complex Molecular Function:protein C-terminus binding; protein binding; gamma-tubulin binding Biological Process: induction of apoptosis via death domain receptors; platelet dense granule organization and biogenesis; melanosome organization and biogenesis; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; anterograde synaptic vesicle transport; positive regulation of transcription from RNA polymerase II promoter; microtubule nucleation; anterograde axon cargo transport; neurite development
NCBI Summary:	This gene encodes a protein with multiple functions. The encoded protein has been found in association with the centrosome, shown to co-localize with gamma-tubulin, and also found to be one of the proteins in the BLOC-1 complex which functions in the formation of lysosome-related organelles. A pseudogene of this gene is located on the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]
UniProt Code:	Q6QNY1
NCBI GenInfo Identifier:	47717129
NCBI Gene ID:	282991
NCBI Accession:	NP_001001342.1
UniProt Secondary Accession:	Q6QNY1,Q9CWG9, Q32WR5,
UniProt Related Accession:	Q6QNY1
Molecular Weight:	11,489 Da
NCBI Full Name:	biogenesis of lysosome-related organelles complex 1 subunit 2 isoform 2
NCBI Synonym Full Names:	biogenesis of lysosomal organelles complex-1, subunit 2
NCBI Official Symbol:	BLOC1S2
NCBI Official Synonym Symbols:	CEAP; BLOS2; CEAP11
NCBI Protein Information:	biogenesis of lysosome-related organelles complex 1 subunit 2; 11 kDa centrosome associated protein; BLOC-1 subunit 2; centrosomal 10 kDa protein; centrosome protein oncogene
UniProt Protein Name:	Biogenesis of lysosome-related organelles complex 1 subunit 2
UniProt Synonym Protein Names:	Centrosome-associated protein
Protein Family:	Biogenesis of lysosome-related organelles complex
UniProt Gene Name:	BLOC1S2
UniProt Entry Name:	BL1S2_HUMAN

	Western blot analysis of extracts of various cell lines, using BLOC1S2 antibody (CAB8007) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using BLOC1S2 antibody (CAB8007) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)