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Anti-CDCA8 Antibody (CAB0675)

SKU:
CAB0675
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
  • Cell Cycle Antibodies 1 Anti-CDCA8 Antibody CAB0675
  • Cell Cycle Antibodies 1 Anti-CDCA8 Antibody CAB0675
  • Cell Cycle Antibodies 1 Anti-CDCA8 Antibody CAB0675
  • Cell Cycle Antibodies 1 Anti-CDCA8 Antibody CAB0675
€139 - €419
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Description

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Antibody Name:Anti-CDCA8 Antibody
Antibody SKU:CAB0675
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human CDCA8
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HL-60, HeLa, MCF7, Mouse spleen
Immunogen:Recombinant protein of human CDCA8
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:55143
Uniprot:Q53HL2
Cellular Location:Chromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, nucleolus, spindle
Calculated MW:31kDa
Observed MW:36-38kDa
Synonyms:CDCA8, BOR, BOREALIN, DasraB, MESRGP, borealin
Background:This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16.
UniProt Protein Function:Borealin: Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. Belongs to the borealin family.
UniProt Protein Details:

Protein type:Cell cycle regulation; Nucleolus

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: intercellular bridge; protein complex; nucleolus; spindle; midbody; nucleus; cytosol; chromosome, pericentric region; chromocenter

Molecular Function:protein binding

Biological Process: cell division; mitotic cell cycle; mitotic metaphase plate congression; chromosome organization and biogenesis

NCBI Summary:This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16. [provided by RefSeq, Apr 2013]
UniProt Code:Q53HL2
NCBI GenInfo Identifier:110832774
NCBI Gene ID:55143
NCBI Accession:Q53HL2.2
UniProt Secondary Accession:Q53HL2,Q53HN1, Q96AM3, Q9NVW5, D3DPT4,
UniProt Related Accession:Q53HL2
Molecular Weight:280
NCBI Full Name:Borealin
NCBI Synonym Full Names:cell division cycle associated 8
NCBI Official Symbol:CDCA8  
NCBI Official Synonym Symbols:BOR; DasraB; MESRGP; BOREALIN  
NCBI Protein Information:borealin; Dasra B; dasra-B; hDasra-B; cell division cycle-associated protein 8; pluripotent embryonic stem cell-related gene 3 protein
UniProt Protein Name:Borealin
UniProt Synonym Protein Names:Cell division cycle-associated protein 8; Dasra-B; hDasra-B; Pluripotent embryonic stem cell-related gene 3 protein
UniProt Gene Name:CDCA8  
UniProt Entry Name:BOREA_HUMAN
Anti-CDCA8 Antibody (CAB0675)Western blot analysis of extracts of various cell lines, using CDCA8 antibody (CAB0675) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-CDCA8 Antibody (CAB0675)Immunohistochemistry of paraffin-embedded mouse brain using CDCA8 antibody (CAB0675) (40x lens).
Anti-CDCA8 Antibody (CAB0675)Immunohistochemistry of paraffin-embedded rat kidney using CDCA8 antibody (CAB0675) (40x lens).
Anti-CDCA8 Antibody (CAB0675)Immunohistochemistry of paraffin-embedded human esophagus using CDCA8 antibody (CAB0675) (40x lens).

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