Anti-C1GALT1 Antibody (CAB12865)

Antibody Name:	Anti-C1GALT1 Antibody
Antibody SKU:	CAB12865
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 194-363 of human C1GALT1 (NP_064541.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 194-363 of human C1GALT1 (NP_064541.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FGRR FKPY VKQG YMSG GAGY VLSK EALK RFVD AFKT DKCT HSSS IEDL ALGR CMEI MNVE AGDS RDTI GKET FHPF VPEH HLIK GYLP RTFW YWNY NYYP PVEG PGCC SDLA VSFH YVDS TTMY ELEY LVYH LRPY GYLY RYQP TLPE RILK EISQ ANKN EDTK VKLG NP
Gene ID:	56913
Uniprot:	Q9NS00
Cellular Location:	Membrane, Single-pass type II membrane protein
Calculated MW:	35kDa/42kDa
Observed MW:	42kDa

Synonyms:	C1GALT1, C1GALT, T-synthase
Background:	The protein encoded by this gene generates the common core 1 O-glycan structure, Gal-beta-1-3GalNAc-R, by the transfer of Gal from UDP-Gal to GalNAc-alpha-1-R. Core 1 is a precursor for many extended mucin-type O-glycans on cell surface and secreted glycoproteins. Studies in mice suggest that this gene plays a key role in thrombopoiesis and kidney homeostasis.

UniProt Protein Function:	C1GALT1: Glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. Plays a central role in many processes, such as angiogenesis, thrombopoiesis and kidney homeostasis development. Belongs to the glycosyltransferase 31 family. Beta3- Gal-T subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 2.4.1.122; Glycan Metabolism - O-glycan biosynthesis; Transferase; Membrane protein, integral Chromosomal Location of Human Ortholog: 7p21.3 Cellular Component: Golgi membrane; integral to membrane Molecular Function:glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase activity; protein binding Biological Process: angiogenesis; kidney development; O-glycan processing; O-glycan processing, core 1
NCBI Summary:	The protein encoded by this gene generates the common core 1 O-glycan structure, Gal-beta-1-3GalNAc-R, by the transfer of Gal from UDP-Gal to GalNAc-alpha-1-R. Core 1 is a precursor for many extended mucin-type O-glycans on cell surface and secreted glycoproteins. Studies in mice suggest that this gene plays a key role in thrombopoiesis and kidney homeostasis.[provided by RefSeq, Sep 2010]
UniProt Code:	Q9NS00
NCBI GenInfo Identifier:	74719147
NCBI Gene ID:	56913
NCBI Accession:	Q9NS00.1
UniProt Secondary Accession:	Q9NS00,Q96QH4, Q9BTU1,
UniProt Related Accession:	Q9NS00
Molecular Weight:	35,935 Da
NCBI Full Name:	Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1
NCBI Synonym Full Names:	core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1
NCBI Official Symbol:	C1GALT1
NCBI Official Synonym Symbols:	C1GALT; T-synthase
NCBI Protein Information:	glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1
UniProt Protein Name:	Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1
UniProt Synonym Protein Names:	B3Gal-T8; Core 1 O-glycan T-synthase; Core 1 UDP-galactose:N-acetylgalactosamine-alpha-R beta 1,3-galactosyltransferase 1; Beta-1,3-galactosyltransferase; Core 1 beta1,3-galactosyltransferase 1; C1GalT1; Core 1 beta3-Gal-T1
Protein Family:	C1GALT1-specific chaperone
UniProt Gene Name:	C1GALT1
UniProt Entry Name:	C1GLT_HUMAN

	Western blot analysis of extracts of mouse kidney, using C1GALT1 antibody (CAB12865) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human lung using C1GALT1 antibody (CAB12865) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using C1GALT1 antibody (CAB12865) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using C1GALT1 antibody (CAB12865) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using C1GALT1 antibody (CAB12865) at dilution of 1:100 (40x lens).

ELISA Kits

Human Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 (C1GALT1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)