Anti-CAMK2G Antibody (CAB3231)

Antibody Name:	Anti-CAMK2G Antibody
Antibody SKU:	CAB3231
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 300-410 of human CAMK2G (NP_751909.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, HeLa, HL-60, 22Rv1, Mouse heart, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 300-410 of human CAMK2G (NP_751909.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LKGA ILTT MLVS RNFS VGRQ SSAP ASPA ASAA GLAG QAAK SLLN KKSD GGVK KRKS SSSV HLME PQTT VVHN ATDG IKGS TESC NTTT EDED LKVR KQEI IKIT EQLI EAI
Gene ID:	818
Uniprot:	Q13555
Cellular Location:	Cytoplasmic side, Peripheral membrane protein, Sarcoplasmic reticulum membrane
Calculated MW:	55kDa/58kDa/59kDa/60kDa/61kDa/62kDa/65kDa
Observed MW:	75kDa/80kDa

Synonyms:

CAMK2G, CAMK, CAMK-II, CAMKG

Background:

The product of this gene is one of the four subunits of an enzyme which belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a gamma chain. Many alternatively spliced transcripts encoding different isoforms have been described but the full-length nature of all the variants has not been determined.

UniProt Protein Function:	CAMK2G: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.17; Protein kinase, CAMK; CAMK group; CAMK2 family Chromosomal Location of Human Ortholog: 10q22 Cellular Component: nucleoplasm; sarcoplasmic reticulum membrane; membrane; plasma membrane; cytosol Molecular Function:calmodulin binding; protein binding; protein homodimerization activity; calcium-dependent protein serine/threonine phosphatase activity; calmodulin-dependent protein kinase activity; ATP binding Biological Process: nervous system development; synaptic transmission; dephosphorylation; regulation of skeletal muscle adaptation; insulin secretion; calcium ion transport; protein amino acid autophosphorylation; cytokine and chemokine mediated signaling pathway; regulation of calcium ion transport; cell differentiation; protein oligomerization; G1/S transition of mitotic cell cycle
UniProt Code:	Q13555
NCBI GenInfo Identifier:	62512173
NCBI Gene ID:
NCBI Accession:	Q13555.3
UniProt Secondary Accession:	Q13555,Q13554,
Molecular Weight:	~ 50~80kDa
NCBI Full Name:	Calcium/calmodulin-dependent protein kinase type II subunit gamma
UniProt Protein Name:	Calcium/calmodulin-dependent protein kinase type II subunit gamma
Protein Family:	Calcium/calmodulin-dependent protein kinase
UniProt Gene Name:	CAMK2G
UniProt Entry Name:	KCC2G_HUMAN

	Western blot analysis of extracts of various cell lines, using CAMK2G antibody (CAB3231) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human stomach using CAMK2G antibody (CAB3231) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat heart using CAMK2G antibody (CAB3231) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using CAMK2G antibody (CAB3231) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using CAMK2G Polyclonal Antibody (CAB3231) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

CAMK2G Antibody (PACO08157)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)