Anti-CNR2 Antibody (CAB1762)

Antibody Name:	Anti-CNR2 Antibody
Antibody SKU:	CAB1762
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CNR2 (NP_001832.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Rat brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CNR2 (NP_001832.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEEC WVTE IANG SKDG LDSN PMKD YMIL SGPQ KTAV AVLC TLLG LLSA LENV AVLY LILS SHQL RRKP SYLF IGSL AGAD FLAS VVFA CSFV NFHV FHGV
Gene ID:	1269
Uniprot:	P34972
Cellular Location:	Cell membrane, Cell projection, Multi-pass membrane protein, Perikaryon, dendrite
Calculated MW:	39kDa
Observed MW:	45kDa

Synonyms:

CNR2, CB-2, CB2, CX5

Background:

The cannabinoid delta-9-tetrahydrocannabinol is the principal psychoactive ingredient of marijuana. The proteins encoded by this gene and the cannabinoid receptor 1 (brain) (CNR1) gene have the characteristics of a guanine nucleotide-binding protein (G-protein)-coupled receptor for cannabinoids. They inhibit adenylate cyclase activity in a dose-dependent, stereoselective, and pertussis toxin-sensitive manner. These proteins have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. The cannabinoid receptors are members of family 1 of the G-protein-coupled receptors.

UniProt Protein Function:	CNR2: Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis. Belongs to the G-protein coupled receptor 1 family.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1 Chromosomal Location of Human Ortholog: 1p36.11 Cellular Component: extrinsic to internal side of plasma membrane; integral to plasma membrane; dendrite; plasma membrane; perikaryon Molecular Function:cannabinoid receptor activity Biological Process: negative regulation of mast cell activation; G-protein signaling, coupled to cyclic nucleotide second messenger; negative regulation of nitric-oxide synthase activity; negative regulation of inflammatory response; response to amphetamine; response to lipopolysaccharide; immune response; negative regulation of action potential; negative regulation of synaptic transmission, GABAergic; inflammatory response; sensory perception of pain
NCBI Summary:	The cannabinoid delta-9-tetrahydrocannabinol is the principal psychoactive ingredient of marijuana. The proteins encoded by this gene and the cannabinoid receptor 1 (brain) (CNR1) gene have the characteristics of a guanine nucleotide-binding protein (G-protein)-coupled receptor for cannabinoids. They inhibit adenylate cyclase activity in a dose-dependent, stereoselective, and pertussis toxin-sensitive manner. These proteins have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. The cannabinoid receptors are members of family 1 of the G-protein-coupled receptors. [provided by RefSeq, Jul 2008]
UniProt Code:	P34972
NCBI GenInfo Identifier:	4502929
NCBI Gene ID:	1269
NCBI Accession:	NP_001832.1
UniProt Secondary Accession:	P34972,Q4VBK8, Q5JRH7, Q6B0G7, Q6NSY0, C6ES44,
UniProt Related Accession:	P34972
Molecular Weight:	39,681 Da
NCBI Full Name:	cannabinoid receptor 2
NCBI Synonym Full Names:	cannabinoid receptor 2 (macrophage)
NCBI Official Symbol:	CNR2
NCBI Official Synonym Symbols:	CB2; CX5; CB-2
NCBI Protein Information:	cannabinoid receptor 2; testis-dominant CNR2 isoform CB2
UniProt Protein Name:	Cannabinoid receptor 2
UniProt Synonym Protein Names:	CX5
UniProt Gene Name:	CNR2
UniProt Entry Name:	CNR2_HUMAN

	Western blot analysis of extracts of various cell lines, using CNR2 antibody (CAB1762) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human breast cancer using CNR2 antibody (CAB1762) at dilution of 1:150 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using CNR2 antibody (CAB1762) at dilution of 1:150 (40x lens).

Antibodies

CNR2 Antibody (PACO00531)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)