Anti-CSF3 Antibody (CAB6178)

Antibody Name:	Anti-CSF3 Antibody
Antibody SKU:	CAB6178
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-207 of human CSF3 (NP_000750.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, 293T, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-207 of human CSF3 (NP_000750.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ATPL GPAS SLPQ SFLL KCLE QVRK IQGD GAAL QEKL VSEC ATYK LCHP EELV LLGH SLGI PWAP LSSC PSQA LQLA GCLS QLHS GLFL YQGL LQAL EGIS PELG PTLD TLQL DVAD FATT IWQQ MEEL GMAP ALQP TQGA MPAF ASAF QRRA GGVL VASH LQSF LEVS YRVL RHLA QP
Gene ID:	1440
Uniprot:	P09919
Cellular Location:	Secreted
Calculated MW:	18kDa/21kDa/22kDa
Observed MW:	22kDa

Synonyms:	CSF3, C17orf33, CSF3OS, GCSF
Background:	The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene.

UniProt Protein Function:	G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cytokine; Secreted, signal peptide; Cell cycle regulation; Secreted Chromosomal Location of Human Ortholog: 17q11.2-q12 Cellular Component: extracellular space Molecular Function:enzyme binding; growth factor activity; cytokine activity; granulocyte colony-stimulating factor receptor binding Biological Process: positive regulation of protein binding; positive regulation of myeloid cell differentiation; granulocyte differentiation; multicellular organismal development; cytokine and chemokine mediated signaling pathway; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; immune response; positive regulation of transcription from RNA polymerase II promoter
NCBI Summary:	The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
UniProt Code:	P09919
NCBI GenInfo Identifier:	117564
NCBI Gene ID:	1440
NCBI Accession:	P09919.1
UniProt Secondary Accession:	P09919,A8MXR7,
UniProt Related Accession:	P09919
Molecular Weight:	18,434 Da
NCBI Full Name:	Granulocyte colony-stimulating factor
NCBI Synonym Full Names:	colony stimulating factor 3 (granulocyte)
NCBI Official Symbol:	CSF3
NCBI Official Synonym Symbols:	GCSF; CSF3OS; C17orf33
NCBI Protein Information:	granulocyte colony-stimulating factor; filgrastim; lenograstim; pluripoietin
UniProt Protein Name:	Granulocyte colony-stimulating factor
UniProt Synonym Protein Names:	Pluripoietin; INN: Filgrastim; INN: Lenograstim
Protein Family:	Granulocyte colony-stimulating factor
UniProt Gene Name:	CSF3
UniProt Entry Name:	CSF3_HUMAN

	Western blot analysis of extracts of various cell lines, using CSF3 antibody (CAB6178) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat spleen using CSF3 antibody (CAB6178) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using CSF3 antibody (CAB6178) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using CSF3 antibody (CAB6178) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-CSF3 Antibody (CAB15660)	Human G-CSF (Granulocyte Colony Stimulating Factor 3) CLIA Kit (HUES00077)
	Human G-CSF (Granulocyte Colony Stimulating Factor 3) ELISA Kit (HUES01366)
	Human Granulocyte colony-stimulating factor (CSF3) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)