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Anti-DGAT2 Antibody (CAB13891)

SKU:
CAB13891
Product Type:
Antibody
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
€139 - €419
Frequently bought together:

Description

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Antibody Name:Anti-DGAT2 Antibody
Antibody SKU:CAB13891
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 113-388 of human DGAT2 (NP_115953.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Rat
Positive Samples:Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 113-388 of human DGAT2 (NP_115953.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DWNT PKKG GRRS QWVR NWAV WRYF RDYF PIQL VKTH NLLT TRNY IFGY HPHG IMGL GAFC NFST EATE VSKK FPGI RPYL ATLA GNFR MPVL REYL MSGG ICPV SRDT IDYL LSKN GSGN AIII VVGG AAES LSSM PGKN AVTL RNRK GFVK LALR HGAD LVPI YSFG ENEV YKQV IFEE GSWG RWVQ KKFQ KYIG FAPC IFHG RGLF SSDT WGLV PYSK PITT VVGE PITI PKLE HPTQ QDID LYHT MYME ALVK LFDK HKTK FGLP ETEV LEVN
Gene ID:84649
Uniprot:Q96PD7
Cellular Location:Endoplasmic reticulum membrane, Lipid droplet, Multi-pass membrane protein
Calculated MW:39kDa/43kDa
Observed MW:44kDa
Synonyms:DGAT2, ARAT, GS1999FULL, HMFN1045
Background:This gene encodes one of two enzymes which catalyzes the final reaction in the synthesis of triglycerides in which diacylglycerol is covalently bound to long chain fatty acyl-CoAs. The encoded protein catalyzes this reaction at low concentrations of magnesium chloride while the other enzyme has high activity at high concentrations of magnesium chloride. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:DGAT2: Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides. Functions also as an acyl-CoA retinol acyltransferase (ARAT). Belongs to the diacylglycerol acyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cofactor and Vitamin Metabolism - retinol; EC 2.3.1.20; Membrane protein, integral; Transferase; Membrane protein, multi-pass; Lipid Metabolism - glycerolipid; EC 2.3.1.76

Chromosomal Location of Human Ortholog: 11q13.5

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; integral to membrane; lipid particle; mitochondrion; perinuclear region of cytoplasm

Molecular Function:diacylglycerol O-acyltransferase activity; protein homodimerization activity

Biological Process: cholesterol homeostasis; diacylglycerol metabolic process; fatty acid homeostasis; sequestering of lipid; triacylglycerol biosynthetic process

NCBI Summary:This gene encodes one of two enzymes which catalyzes the final reaction in the synthesis of triglycerides in which diacylglycerol is covalently bound to long chain fatty acyl-CoAs. The encoded protein catalyzes this reaction at low concentrations of magnesium chloride while the other enzyme has high activity at high concentrations of magnesium chloride. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
UniProt Code:Q96PD7
NCBI GenInfo Identifier:74732654
NCBI Gene ID:84649
NCBI Accession:Q96PD7.2
UniProt Secondary Accession:Q96PD7,Q5U810, Q68CL3, Q68DJ0, Q8NDB7, Q96BS0, Q9BYE5 A6ND76,
UniProt Related Accession:Q96PD7
Molecular Weight:39,044 Da
NCBI Full Name:Diacylglycerol O-acyltransferase 2
NCBI Synonym Full Names:diacylglycerol O-acyltransferase 2
NCBI Official Symbol:DGAT2  
NCBI Official Synonym Symbols:ARAT; HMFN1045; GS1999FULL  
NCBI Protein Information:diacylglycerol O-acyltransferase 2
UniProt Protein Name:Diacylglycerol O-acyltransferase 2
UniProt Synonym Protein Names:Acyl-CoA retinol O-fatty-acyltransferase (EC:2.3.1.76); ARAT; Retinol O-fatty-acyltransferase; Diglyceride acyltransferase 2
Protein Family:Diacylglycerol O-acyltransferase
UniProt Gene Name:DGAT2  
UniProt Entry Name:DGAT2_HUMAN
Anti-DGAT2 Antibody (CAB13891)Western blot analysis of extracts of Rat liver cells, using DGAT2 antibody (CAB13891) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 60s.

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