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Anti-DHODH Antibody (CAB6899)

SKU:
CAB6899
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
  • Metabolism Antibodies 2 Anti-DHODH Antibody CAB6899
  • Metabolism Antibodies 2 Anti-DHODH Antibody CAB6899
  • Metabolism Antibodies 2 Anti-DHODH Antibody CAB6899
  • Metabolism Antibodies 2 Anti-DHODH Antibody CAB6899
€139 - €419
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Description

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Antibody Name:Anti-DHODH Antibody
Antibody SKU:CAB6899
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 116-395 of human DHODH (NP_001352.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, HL-60, SW620, HepG2, Mouse heart, Mouse spleen, Mouse kidney, Rat heart, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 116-395 of human DHODH (NP_001352.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EIGS VTPK PQEG NPRP RVFR LPED QAVI NRYG FNSH GLSV VEHR LRAR QQKQ AKLT EDGL PLGV NLGK NKTS VDAA EDYA EGVR VLGP LADY LVVN VSSP NTAG LRSL QGKA ELRR LLTK VLQE RDGL RRVH RPAV LVKI APDL TSQD KEDI ASVV KELG IDGL IVTN TTVS RPAG LQGA LRSE TGGL SGKP LRDL STQT IREM YALT QGRV PIIG VGGV SSGQ DALE KIRA GASL VQLY TALT FWGP PVVG KVKR ELEA LLKE QGFG GVTD AIGA DHRR
Gene ID:1723
Uniprot:Q02127
Cellular Location:Mitochondrion inner membrane, Single-pass membrane protein
Calculated MW:42kDa
Observed MW:43kDa
Synonyms:DHODH, DHOdehase, POADS, URA1
Background:The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane.
UniProt Protein Function:DHODH: Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS); also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases. Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.
UniProt Protein Details:

Protein type:Mitochondrial; EC 1.3.5.2; Nucleotide Metabolism - pyrimidine; Membrane protein, integral; Oxidoreductase

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: cytoplasm; mitochondrial inner membrane; mitochondrion; nucleoplasm

Molecular Function:dihydroorotate dehydrogenase activity

Biological Process: 'de novo' pyrimidine base biosynthetic process; pyrimidine nucleoside biosynthetic process

Disease: Postaxial Acrofacial Dysostosis

NCBI Summary:The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. [provided by RefSeq, Jul 2008]
UniProt Code:Q02127
NCBI GenInfo Identifier:56405372
NCBI Gene ID:1723
NCBI Accession:Q02127.3
UniProt Secondary Accession:Q02127,Q6P176, A8K8C8,
UniProt Related Accession:Q02127
Molecular Weight:42,867 Da
NCBI Full Name:Dihydroorotate dehydrogenase (quinone), mitochondrial
NCBI Synonym Full Names:dihydroorotate dehydrogenase (quinone)
NCBI Official Symbol:DHODH  
NCBI Official Synonym Symbols:URA1; POADS; DHOdehase  
NCBI Protein Information:dihydroorotate dehydrogenase (quinone), mitochondrial
UniProt Protein Name:Dihydroorotate dehydrogenase (quinone), mitochondrial
UniProt Synonym Protein Names:Dihydroorotate oxidase
UniProt Gene Name:DHODH  
UniProt Entry Name:PYRD_HUMAN
Anti-DHODH Antibody (CAB6899)Western blot analysis of extracts of various cell lines, using DHODH antibody (CAB6899) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-DHODH Antibody (CAB6899)Immunohistochemistry of paraffin-embedded human liver cancer using DHODH antibody (CAB6899) at dilution of 1:100 (40x lens).
Anti-DHODH Antibody (CAB6899)Immunohistochemistry of paraffin-embedded human esophagus using DHODH antibody (CAB6899) at dilution of 1:100 (40x lens).
Anti-DHODH Antibody (CAB6899)Immunohistochemistry of paraffin-embedded mouse brain using DHODH antibody (CAB6899) at dilution of 1:100 (40x lens).

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