Anti-EDA Antibody (CAB2905)

Antibody Name:	Anti-EDA Antibody
Antibody SKU:	CAB2905
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human EDA

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, HepG2, BxPC-3, Mouse liver

Immunogen:	A synthetic peptide of human EDA
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	1896
Uniprot:	Q92838
Cellular Location:	Cell membrane, Secreted, Single-pass type II membrane protein
Calculated MW:	14kDa/15kDa/40kDa/41kDa
Observed MW:	48kDa

Synonyms:

EDA, ECTD1, ED1, ED1-A1, ED1-A2, EDA-A1, EDA-A2, EDA1, EDA2, HED, HED1, ODT1, STHAGX1, TNLG7C, XHED, XLHED

Background:

The protein encoded by this gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in this gene are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia. Several transcript variants encoding many different isoforms have been found for this gene.

UniProt Protein Function:	EDA: Seems to be involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Isoform 1 binds only to the receptor EDAR, while isoform 3 binds exclusively to the receptor XEDAR. Defects in EDA are the cause of ectodermal dysplasia type 1 (ED1); also known as Christ-Siemens-Touraine syndrome or X-linked hypohidrotic ectodermal dysplasia (XLHED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ED1 is a disease characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands. ED1 is the most common form of over 150 clinically distinct ectodermal dysplasias. Defects in EDA are the cause of tooth agenesis selective X-linked type 1 (STHAGX1). A form of selective tooth agenesis, a common anomaly characterized by the congenital absence of one or more teeth. Selective tooth agenesis without associated systemic disorders has sometimes been divided into 2 types: oligodontia, defined as agenesis of 6 or more permanent teeth, and hypodontia, defined as agenesis of less than 6 teeth. The number in both cases does not include absence of third molars (wisdom teeth). Belongs to the tumor necrosis factor family. 8 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Receptor, misc.; Motility/polarity/chemotaxis; Membrane protein, integral Chromosomal Location of Human Ortholog: Xq12-q13.1 Cellular Component: endoplasmic reticulum membrane; collagen; cytoskeleton; membrane; intracellular membrane-bound organelle; apical part of cell; integral to plasma membrane; integral to membrane; plasma membrane; extracellular region Molecular Function:protein binding; tumor necrosis factor receptor binding; receptor binding Biological Process: pigmentation; cell-matrix adhesion; ectoderm development; immune response; positive regulation of NF-kappaB import into nucleus; gene expression; cell differentiation; signal transduction; activation of NF-kappaB transcription factor; odontogenesis of dentine-containing teeth Disease: Tooth Agenesis, Selective, X-linked, 1; Ectodermal Dysplasia 1, Hypohidrotic, X-linked
NCBI Summary:	The protein encoded by this gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in this gene are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia. Several transcript variants encoding many different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q92838
NCBI GenInfo Identifier:	6166135
NCBI Gene ID:	1896
NCBI Accession:	Q92838.2
UniProt Secondary Accession:	Q92838,O75910, Q5JS00, Q5JUM7, Q9UP77, Q9Y6L0, Q9Y6L1 Q9Y6L2, A0AUZ2, A2A337, B7ZLU2, B7ZLU4,
UniProt Related Accession:	Q92838
Molecular Weight:	40,750 Da
NCBI Full Name:	Ectodysplasin-A
NCBI Synonym Full Names:	ectodysplasin A
NCBI Official Symbol:	EDA
NCBI Official Synonym Symbols:	ED1; HED; EDA1; EDA2; HED1; ODT1; XHED; ECTD1; XLHED; ED1-A1; ED1-A2; EDA-A1; EDA-A2; STHAGX1
NCBI Protein Information:	ectodysplasin-A; oligodontia 1; X-linked anhidroitic ectodermal dysplasia protein
UniProt Protein Name:	Ectodysplasin-A
UniProt Synonym Protein Names:	Ectodermal dysplasia protein; EDA protein
Protein Family:	Ectodysplasin
UniProt Gene Name:	EDA
UniProt Entry Name:	EDA_HUMAN

	Western blot analysis of extracts of various cell lines, using EDA antibody (CAB2905) at 1:400 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human uterine cancer using EDA Antibody (CAB2905) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded rat liver using EDA Antibody (CAB2905) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using EDA Antibody (CAB2905) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
EDA Antibody (PACO07220)	Human EDA / Ectodysplasin-A ELISA Kit
	Human Ectodysplasin-A (EDA) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)