Anti-FA2H Antibody (CAB13873)

Antibody Name:	Anti-FA2H Antibody
Antibody SKU:	CAB13873
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 95-170 of human FA2H (NP_077282.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	U-87MG, HT-29, SGC-7901, BxPC-3, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 95-170 of human FA2H (NP_077282.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NEPV ALEE TQKT DPAM EPRF KVVD WDKD LVDW RKPL LWQV GHLG EKYD EWVH QPVT RPIR LFHS DLIE GLSK TVWY
Gene ID:	79152
Uniprot:	Q7L5A8
Cellular Location:	Endoplasmic reticulum membrane, Microsome membrane, Multi-pass membrane protein
Calculated MW:	18kDa/42kDa
Observed MW:	38kDa

Synonyms:

FA2H, FAAH, FAH1, FAXDC1, SCS7, SPG35

Background:

This gene encodes a protein that catalyzes the synthesis of 2-hydroxysphingolipids, a subset of sphingolipids that contain 2-hydroxy fatty acids. Sphingolipids play roles in many cellular processes and their structural diversity arises from modification of the hydrophobic ceramide moiety, such as by 2-hydroxylation of the N-acyl chain, and the existence of many different head groups. Mutations in this gene have been associated with leukodystrophy dysmyelinating with spastic paraparesis with or without dystonia.

UniProt Protein Function:	FA2H: Required for alpha-hydroxylation of free fatty acids and the formation of alpha-hydroxylated sphingolipids. Defects in FA2H are a cause of spastic paraplegia autosomal recessive type 35 (SPG35). Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Belongs to the sterol desaturase family. SCS7 subfamily.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; EC 1.-.-.-; Membrane protein, integral; Oxidoreductase Chromosomal Location of Human Ortholog: 16q23 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane Biological Process: fatty acid metabolic process; sphingolipid biosynthetic process Disease: Spastic Paraplegia 35, Autosomal Recessive
NCBI Summary:	This gene encodes a protein that catalyzes the synthesis of 2-hydroxysphingolipids, a subset of sphingolipids that contain 2-hydroxy fatty acids. Sphingolipids play roles in many cellular processes and their structural diversity arises from modification of the hydrophobic ceramide moiety, such as by 2-hydroxylation of the N-acyl chain, and the existence of many different head groups. Mutations in this gene have been associated with leukodystrophy dysmyelinating with spastic paraparesis with or without dystonia.[provided by RefSeq, Mar 2010]
UniProt Code:	Q7L5A8
NCBI GenInfo Identifier:	74749893
NCBI Gene ID:	79152
NCBI Accession:	Q7L5A8.1
UniProt Secondary Accession:	Q7L5A8,O75213, Q96DK1, Q9H1A5, B7Z8T6,
UniProt Related Accession:	Q7L5A8
Molecular Weight:	18,324 Da
NCBI Full Name:	Fatty acid 2-hydroxylase
NCBI Synonym Full Names:	fatty acid 2-hydroxylase
NCBI Official Symbol:	FA2H
NCBI Official Synonym Symbols:	FAAH; FAH1; SCS7; SPG35; FAXDC1
NCBI Protein Information:	fatty acid 2-hydroxylase
UniProt Protein Name:	Fatty acid 2-hydroxylase
UniProt Synonym Protein Names:	Fatty acid alpha-hydroxylase
Protein Family:	Fatty acid 2-hydroxylase
UniProt Gene Name:	FA2H
UniProt Entry Name:	FA2H_HUMAN

	Western blot analysis of extracts of various cell lines, using FA2H antibody (CAB13873) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human stomach using FA2H antibody (CAB13873) at dilution of 1:150 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using FA2H antibody (CAB13873) at dilution of 1:150 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using FA2H antibody (CAB13873) at dilution of 1:150 (40x lens).

Antibodies

Anti-FA2H Antibody (CAB13874)

FA2H Antibody (PACO07440)

FA2H Antibody (PACO09183)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)