Anti-FMOD Antibody (CAB6375)

Antibody Name:	Anti-FMOD Antibody
Antibody SKU:	CAB6375
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-376 of human FMOD (NP_002014.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, Mouse spleen, Rat spleen, Rat skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-376 of human FMOD (NP_002014.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QYED DPHW WFHY LRSQ QSTY YDPY DPYP YETY EPYP YGVD EGPA YTYG SPSP PDPR DCPQ ECDC PPNF PTAM YCDN RNLK YLPF VPSR MKYV YFQN NQIT SIQE GVFD NATG LLWI ALHG NQIT SDKV GRKV FSKL RHLE RLYL DHNN LTRM PGPL PRSL RELH LDHN QISR VPNN ALEG LENL TALY LQHN EIQE VGSS MRGL RSLI LLDL SYNH LRKV PDGL PSAL EQLY MEHN NVYT VPDS YFRG APKL LYVR LSHN SLTN NGLA SNTF NSSS LLEL DLSY NQLQ KIPP VNTN LENL YLQG NRIN EFSI SSFC TVVD VVNF SKLQ VLRL DGNE IKRS AMPA DAPL CLRL ASLI EI
Gene ID:	2331
Uniprot:	Q06828
Cellular Location:	Secreted, extracellular matrix, extracellular space
Calculated MW:	43kDa
Observed MW:	45kDa

Synonyms:

FMOD, FM, SLRR2E

Background:

Fibromodulin belongs to the family of small interstitial proteoglycans. The encoded protein possesses a central region containing leucine-rich repeats with 4 keratan sulfate chains, flanked by terminal domains containing disulphide bonds. Owing to the interaction with type I and type II collagen fibrils and in vitro inhibition of fibrillogenesis, the encoded protein may play a role in the assembly of extracellular matrix. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix. Sequence variations in this gene may be associated with the pathogenesis of high myopia. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	FMOD: Affects the rate of fibrils formation. May have a primary role in collagen fibrillogenesis. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 1q32 Cellular Component: extracellular matrix; extracellular region; extracellular space; Golgi lumen; lysosomal lumen Molecular Function:heparin binding; Roundabout binding Biological Process: axon extension involved in axon guidance; axonogenesis; keratan sulfate biosynthetic process; keratan sulfate catabolic process; regulation of axonogenesis; transforming growth factor beta receptor complex assembly
NCBI Summary:	Fibromodulin belongs to the family of small interstitial proteoglycans. The encoded protein possesses a central region containing leucine-rich repeats with 4 keratan sulfate chains, flanked by terminal domains containing disulphide bonds. Owing to the interaction with type I and type II collagen fibrils and in vitro inhibition of fibrillogenesis, the encoded protein may play a role in the assembly of extracellular matrix. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix. Sequence variations in this gene may be associated with the pathogenesis of high myopia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
UniProt Code:	Q06828
NCBI GenInfo Identifier:	223590208
NCBI Gene ID:	2331
NCBI Accession:	Q06828.2
UniProt Secondary Accession:	Q06828,Q15331, Q8IV47,
UniProt Related Accession:	Q06828
Molecular Weight:	43,179 Da
NCBI Full Name:	Fibromodulin
NCBI Synonym Full Names:	fibromodulin
NCBI Official Symbol:	FMOD
NCBI Official Synonym Symbols:	FM; SLRR2E
NCBI Protein Information:	fibromodulin
UniProt Protein Name:	Fibromodulin
UniProt Synonym Protein Names:	Collagen-binding 59 kDa protein; Keratan sulfate proteoglycan fibromodulin; KSPG fibromodulin
Protein Family:	Fibromodulin
UniProt Gene Name:	FMOD
UniProt Entry Name:	FMOD_HUMAN

	Western blot analysis of extracts of various cell lines, using FMOD antibody (CAB6375) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat testis using FMOD antibody (CAB6375) at dilution of 1:100 (20x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using FMOD antibody (CAB6375) at dilution of 1:100 (20x lens).

Antibodies	ELISA Kits
FMOD Antibody (PACO09325)	Human Fibromodulin / FMOD ELISA Kit
	Human FMOD (Fibromodulin) CLIA Kit (HUES01119)
	Human Fibromodulin (FMOD) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)