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Anti-GABRA1 Antibody (CAB12244)

SKU:
CAB12244
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction
€139 - €419
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Description

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Antibody Name:Anti-GABRA1 Antibody
Antibody SKU:CAB12244
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 28-251 of human GABRA1 (NP_000797.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Mouse
Positive Samples:Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 28-251 of human GABRA1 (NP_000797.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QPSL QDEL KDNT TVFT RILD RLLD GYDN RLRP GLGE RVTE VKTD IFVT SFGP VSDH DMEY TIDV FFRQ SWKD ERLK FKGP MTVL RLNN LMAS KIWT PDTF FHNG KKSV AHNM TMPN KLLR ITED GTLL YTMR LTVR AECP MHLE DFPM DAHA CPLK FGSY AYTR AEVV YEWT REPA RSVV VAED GSRL NQYD LLGQ TVDS GIVQ SSTG EYVV MTTH FHLK RKIG
Gene ID:2554
Uniprot:P14867
Cellular Location:Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Calculated MW:51kDa
Observed MW:52kDa
Synonyms:GABRA1, ECA4, EIEE19, EJM, EJM5
Background:This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene cause juvenile myoclonic epilepsy and childhood absence epilepsy type 4. Multiple transcript variants encoding the same protein have been identified for this gene.
UniProt Protein Function:GABRA1: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4). A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood. Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5). A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.
UniProt Protein Details:

Protein type:Membrane protein, integral; Channel, ligand-gated; Transporter, ion channel; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: postsynaptic membrane; integral to plasma membrane; plasma membrane; cell junction

Molecular Function:chloride channel activity; GABA-A receptor activity; GABA receptor activity; drug binding; extracellular ligand-gated ion channel activity

Biological Process: synaptic transmission; transport; synaptic transmission, GABAergic; transmembrane transport; gamma-aminobutyric acid signaling pathway

Disease: Epilepsy, Idiopathic Generalized, Susceptibility To, 13; Epileptic Encephalopathy, Early Infantile, 19

NCBI Summary:This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene cause juvenile myoclonic epilepsy and childhood absence epilepsy type 4. Multiple transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P14867
NCBI GenInfo Identifier:27808653
NCBI Gene ID:2554
NCBI Accession:P14867.3
UniProt Secondary Accession:P14867,Q8N629, D3DQK6,
UniProt Related Accession:P14867
Molecular Weight:51,802 Da
NCBI Full Name:Gamma-aminobutyric acid receptor subunit alpha-1
NCBI Synonym Full Names:gamma-aminobutyric acid (GABA) A receptor, alpha 1
NCBI Official Symbol:GABRA1  
NCBI Official Synonym Symbols:EJM; ECA4; EJM5; EIEE19  
NCBI Protein Information:gamma-aminobutyric acid receptor subunit alpha-1; GABA(A) receptor, alpha 1; GABA(A) receptor subunit alpha-1
UniProt Protein Name:Gamma-aminobutyric acid receptor subunit alpha-1
UniProt Synonym Protein Names:GABA(A) receptor subunit alpha-1
UniProt Gene Name:GABRA1  
UniProt Entry Name:GBRA1_HUMAN
Anti-GABRA1 Antibody (CAB12244)Western blot analysis of extracts of mouse brain, using GABRA1 antibody (CAB12244) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

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