Anti-GCA Antibody (CAB7086)

Antibody Name:	Anti-GCA Antibody
Antibody SKU:	CAB7086
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human GCA (NP_036330.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	HL-60, HT-29, Mouse kidney, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human GCA (NP_036330.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAYP GYGG GFGN FSIQ VPGM QMGQ PVPE TGPA ILLD GYSG PAYS DTYS SAGD SVYT YFSA VAGQ DGEV DAEE LQRC LTQS GING TYSP FSLE TCRI MIAM LDRD HTGK MGFN AFKE LWAA LNAW KENF MTVD QDGS GTVE HHEL RQAI GLMG YRLS PQTL TTIV KRYS KNGR IFFD DYVA CCVK LRAL TDFF RKRD HLQQ GSAN FIYD DFLQ GTMA I
Gene ID:	25801
Uniprot:	P28676
Cellular Location:	Cytoplasm, Cytoplasmic granule membrane, Cytoplasmic side, Peripheral membrane protein
Calculated MW:	24kDa
Observed MW:	24kDa

Synonyms:

GCA, GCL

Background:

This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.

UniProt Protein Function:	GCA: Calcium-binding protein that may play a role in the adhesion of neutrophils to fibronectin. May play a role in the formation of focal adhesions.
UniProt Protein Details:	Protein type:Calcium-binding Chromosomal Location of Human Ortholog: 2q24.2 Cellular Component: cytoplasm; plasma membrane Molecular Function:protein homodimerization activity; protein heterodimerization activity; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding Biological Process: proteolysis
NCBI Summary:	This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation. [provided by RefSeq, Jul 2008]
UniProt Code:	P28676
NCBI GenInfo Identifier:	1170014
NCBI Gene ID:	25801
NCBI Accession:	P28676.2
UniProt Secondary Accession:	P28676,Q53TB5, Q59EP3, B2R5X3,
UniProt Related Accession:	P28676
Molecular Weight:	217
NCBI Full Name:	Grancalcin
NCBI Synonym Full Names:	grancalcin, EF-hand calcium binding protein
NCBI Official Symbol:	GCA
NCBI Official Synonym Symbols:	GCL
NCBI Protein Information:	grancalcin; grancalcin, penta-EF-hand protein; grancalcin, EF-hand calcium-binding protein
UniProt Protein Name:	Grancalcin
Protein Family:	Grancalcin
UniProt Gene Name:	GCA
UniProt Entry Name:	GRAN_HUMAN

	Western blot analysis of extracts of various cell lines, using GCA antibody (CAB7086) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of A549 cells using GCA antibody (CAB7086).

ELISA Kits

Human GCA (Grancalcin) ELISA Kit (HUFI05170)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)