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Anti-GRN Antibody (CAB5773)

SKU:
CAB5773
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

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Antibody Name:Anti-GRN Antibody
Antibody SKU:CAB5773
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-310 of human GRN (NP_002078.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HL-60, THP-1, PC-12, 293T, HepG2, MCF7, Mouse liver, Mouse spleen, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-310 of human GRN (NP_002078.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:CPDG QFCP VACC LDPG GASY SCCR PLLD KWPT TLSR HLGG PCQV DAHC SAGH SCIF TVSG TSSC CPFP EAVA CGDG HHCC PRGF HCSA DGRS CFQR SGNN SVGA IQCP DSQF ECPD FSTC CVMV DGSW GCCP MPQA SCCE DRVH CCPH GAFC DLVH TRCI TPTG THPL AKKL PAQR TNRA VALS SSVM CPDA RSRC PDGS TCCE LPSG KYGC CPMP NATC CSDH LHCC PQDT VCDL IQSK CLSK ENAT TDLL TKLP AHTV GDVK CDME VSCP DGYT CCRL QSGA WGCC PFT
Gene ID:2896
Uniprot:P28799
Cellular Location:Secreted
Calculated MW:44kDa/46kDa/63kDa
Observed MW:50-70kDa
Synonyms:GRN, CLN11, GEP, GP88, PCDGF, PEPI, PGRN, granulins
Background:Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.
UniProt Protein Function:GRN: Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD); also known as tau- negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease. Defects in GRN are the cause of neuronal ceroid lipofuscinosis type 11 (CLN11). A form of neuronal ceroid lipofuscinosis characterized by rapidly progressive visual loss due to retinal dystrophy, seizures, cerebellar ataxia, and cerebellar atrophy. Cognitive decline may also occur. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material. Belongs to the granulin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: intracellular membrane-bound organelle

Molecular Function:growth factor activity; protein binding

Disease: Ceroid Lipofuscinosis, Neuronal, 11; Frontotemporal Lobar Degeneration With Tdp43 Inclusions, Grn-related

NCBI Summary:Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. [provided by RefSeq, Jul 2008]
UniProt Code:P28799
NCBI GenInfo Identifier:77416865
NCBI Gene ID:2896
NCBI Accession:P28799.2
UniProt Secondary Accession:P28799,P23781, P23782, P23783, P23784, Q53HQ8, Q53Y88 Q540U8, Q9BWE7, Q9H8S1, Q9UCH0, D3DX55,
UniProt Related Accession:P28799
Molecular Weight:44,132 Da
NCBI Full Name:Granulins
NCBI Synonym Full Names:granulin
NCBI Official Symbol:GRN  
NCBI Official Synonym Symbols:GEP; GP88; PEPI; PGRN; CLN11; PCDGF  
NCBI Protein Information:granulins
UniProt Protein Name:Granulins
UniProt Synonym Protein Names:Proepithelin; PEPI
Protein Family:Granulin
UniProt Gene Name:GRN  
UniProt Entry Name:GRN_HUMAN
Anti-GRN Antibody (CAB5773)Western blot analysis of extracts of various cell lines, using GRN antibody (CAB5773) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5s.

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