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Anti-HLA-DMA Antibody (CAB6922)

SKU:
CAB6922
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
€139 - €419
Frequently bought together:

Description

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Antibody Name:Anti-HLA-DMA Antibody
Antibody SKU:CAB6922
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 27-233 of human HLA-DMA (NP_006111.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:A-431, Jurkat, HeLa, Mouse lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 27-233 of human HLA-DMA (NP_006111.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VPEA PTPM WPDD LQNH TFLH TVYC QDGS PSVG LSEA YDED QLFF FDFS QNTR VPRL PEFA DWAQ EQGD APAI LFDK EFCE WMIQ QIGP KLDG KIPV SRGF PIAE VFTL KPLE FGKP NTLV CFVS NLFP PMLT VNWQ HHSV PVEG FGPT FVSA VDGL SFQA FSYL NFTP EPSD IFSC IVTH EIDR YTAI AYWV PRNA LPSD LLEN VLC
Gene ID:3108
Uniprot:P28067
Cellular Location:Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein
Calculated MW:29kDa
Observed MW:35kDa
Synonyms:HLA-DMA, D6S222E, DMA, HLADM, RING6, major histocompatibility complex, class II, DM alpha
Background:HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail.
UniProt Protein Function:Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO.
NCBI Summary:HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. [provided by RefSeq, Jul 2008]
UniProt Code:P28067
NCBI GenInfo Identifier:133158
NCBI Gene ID:3108
NCBI Accession:P28067.1
UniProt Secondary Accession:P28067,Q29639, Q29640,
UniProt Related Accession:P28067
Molecular Weight:28
NCBI Full Name:HLA class II histocompatibility antigen, DM alpha chain
NCBI Synonym Full Names:major histocompatibility complex, class II, DM alpha
NCBI Official Symbol:HLA-DMA  
NCBI Official Synonym Symbols:DMA; HLADM; RING6; D6S222E  
NCBI Protein Information:HLA class II histocompatibility antigen, DM alpha chain
UniProt Protein Name:HLA class II histocompatibility antigen, DM alpha chain
UniProt Synonym Protein Names:MHC class II antigen DMA; Really interesting new gene 6 protein
UniProt Gene Name:HLA-DMA  
Anti-HLA-DMA Antibody (CAB6922)Western blot analysis of extracts of various cell lines, using HLA-DMA antibody (CAB6922) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

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