Anti-IL-11 Antibody (CAB1902)

Antibody Name:	IL11 Rabbit Polyclonal Antibody
Antibody SKU:	CAB1902
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-199 of human IL11 (NP_000632.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Human plasma

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-199 of human IL11 (NP_000632.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PGPP PGPP RVSP DPRA ELDS TVLL TRSL LADT RQLA AQLR DKFP ADGD HNLD SLPT LAMS AGAL GALQ LPGV LTRL RADL LSYL RHVQ WLRR AGGS SLKT LEPE LGTL QARL DRLL RRLQ LLMS RLAL PQPP PDPP APPL APPS SAWG GIRA AHAI LGGL HLTL DWAV RGLL LLKT RL
Gene ID:	3589
Uniprot:	P20809
Cellular Location:	Secreted
Calculated MW:	12kDa/21kDa
Observed MW:	21kDa

Synonyms:

IL11, AGIF, IL-11

Background:

The protein encoded by this gene is a member of the gp130 family of cytokines. These cytokines drive the assembly of multisubunit receptor complexes, all of which contain at least one molecule of the transmembrane signaling receptor IL6ST (gp130). This cytokine is shown to stimulate the T-cell-dependent development of immunoglobulin-producing B cells. It is also found to support the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

UniProt Protein Function:	IL11: Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Belongs to the IL-6 superfamily.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Cytokine; Cell cycle regulation; Secreted, signal peptide Chromosomal Location of Human Ortholog: 19q13.3-q13.4 Cellular Component: extracellular space; cytoplasm; extracellular region Molecular Function:growth factor activity; cytokine activity; interleukin-11 receptor binding Biological Process: fat cell differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of MAPKKK cascade; cell-cell signaling; megakaryocyte differentiation; B cell differentiation; negative regulation of hormone secretion; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation
NCBI Summary:	The protein encoded by this gene is a member of the gp130 family of cytokines. These cytokines drive the assembly of multisubunit receptor complexes, all of which contain at least one molecule of the transmembrane signaling receptor IL6ST (gp130). This cytokine is shown to stimulate the T-cell-dependent development of immunoglobulin-producing B cells. It is also found to support the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2012]
UniProt Code:	P20809
NCBI GenInfo Identifier:	124294
NCBI Gene ID:	3589
NCBI Accession:	P20809.1
UniProt Secondary Accession:	P20809,Q96EB4, B4DQV5,
UniProt Related Accession:	P20809
Molecular Weight:	12,994 Da
NCBI Full Name:	Interleukin-11
NCBI Synonym Full Names:	interleukin 11
NCBI Official Symbol:	IL11
NCBI Official Synonym Symbols:	AGIF; IL-11
NCBI Protein Information:	interleukin-11; oprelvekin; adipogenesis inhibitory factor
UniProt Protein Name:	Interleukin-11
UniProt Synonym Protein Names:	Adipogenesis inhibitory factor; AGIF; INN: Oprelvekin
Protein Family:	Interleukin
UniProt Gene Name:	IL11
UniProt Entry Name:	IL11_HUMAN

	Western blot analysis of extracts of Human plasma, using IL11 antibody (CAB1902) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded Mouse kidney using IL11 Rabbit pAb (CAB1902) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat lung using IL11 Rabbit pAb (CAB1902) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human esophageal using IL11 Rabbit pAb (CAB1902) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using IL11 antibody (CAB1902) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
IL11 Antibody (PACO04159)	Human IL-11 CLIA Kit (HUES01285)
	Human IL-11 ELISA Kit (HUEB0017)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)