Anti-IL27RA Antibody (CAB8176)

Antibody Name:	IL27RA Rabbit Polyclonal Antibody
Antibody SKU:	CAB8176
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 33-283 of human IL27RA (NP_004834.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 33-283 of human IL27RA (NP_004834.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QGSA GPLQ CYGV GPLG DLNC SWEP LGDL GAPS ELHL QSQK YRSN KTQT VAVA AGRS WVAI PREQ LTMS DKLL VWGT KAGQ PLWP PVFV NLET QMKP NAPR LGPD VDFS EDDP LEAT VHWA PPTW PSHK VLIC QFHY RRCQ EAAW TLLE PELK TIPL TPVE IQDL ELAT GYKV YGRC RMEK EEDL WGEW SPIL SFQT PPSA PKDV WVSG NLCG TPGG EEPL LLWK APGP CVQV SYKV WFWV GGRE LSPE GIT
Gene ID:	9466
Uniprot:	Q6UWB1
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	69kDa
Observed MW:	75kDa

Synonyms:

IL27RA, CRL1, IL-27RA, IL27R, TCCR, WSX1, zcytor1

Background:

In mice, CD4+ helper T-cells differentiate into type 1 (Th1) cells, which are critical for cell-mediated immunity, predominantly under the influence of IL12. Also, IL4 influences their differentiation into type 2 (Th2) cells, which are critical for most antibody responses. Mice deficient in these cytokines, their receptors, or associated transcription factors have impaired, but are not absent of, Th1 or Th2 immune responses. This gene encodes a protein which is similar to the mouse T-cell cytokine receptor Tccr at the amino acid level, and is predicted to be a glycosylated transmembrane protein.

UniProt Protein Function:	IL27RA: Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1- type immune responses. Also appears to be involved in innate defense mechanisms. Belongs to the type I cytokine receptor family. Type 2 subfamily.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 19p13.11 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:transmembrane receptor activity Biological Process: cell surface receptor linked signal transduction; immune response
NCBI Summary:	In mice, CD4+ helper T-cells differentiate into type 1 (Th1) cells, which are critical for cell-mediated immunity, predominantly under the influence of IL12. Also, IL4 influences their differentiation into type 2 (Th2) cells, which are critical for most antibody responses. Mice deficient in these cytokines, their receptors, or associated transcription factors have impaired, but are not absent of, Th1 or Th2 immune responses. This gene encodes a protein which is similar to the mouse T-cell cytokine receptor Tccr at the amino acid level, and is predicted to be a glycosylated transmembrane protein. [provided by RefSeq, Jul 2008]
UniProt Code:	Q6UWB1
NCBI GenInfo Identifier:	116242518
NCBI Gene ID:	9466
NCBI Accession:	Q6UWB1.2
UniProt Secondary Accession:	Q6UWB1,O60624, A0N0L1,
UniProt Related Accession:	Q6UWB1
Molecular Weight:	69,474 Da
NCBI Full Name:	Interleukin-27 receptor subunit alpha
NCBI Synonym Full Names:	interleukin 27 receptor subunit alpha
NCBI Official Symbol:	IL27RA
NCBI Official Synonym Symbols:	CRL1; TCCR; WSX1; IL27R; IL-27RA; zcytor1
NCBI Protein Information:	interleukin-27 receptor subunit alpha
UniProt Protein Name:	Interleukin-27 receptor subunit alpha
UniProt Synonym Protein Names:	Cytokine receptor WSX-1; Cytokine receptor-like 1; Type I T-cell cytokine receptor; TCCR; ZcytoR1
Protein Family:	Interleukin-27 receptor
UniProt Gene Name:	IL27RA
UniProt Entry Name:	I27RA_HUMAN

	Western blot analysis of extracts of various cell lines, using IL27RA antibody (CAB8176) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using IL27RA antibody (CAB8176) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)