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Anti-IL5RA Antibody (CAB8552)

SKU:
CAB8552
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€139 - €419
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Description

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Antibody Name:Anti-IL5RA Antibody
Antibody SKU:CAB8552
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-335 of human IL5RA (NP_783855.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HL-60, B cells, U-87MG, Mouse liver, Mouse lung, Rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-335 of human IL5RA (NP_783855.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ADLL PDEK ISLL PPVN FTIK VTGL AQVL LQWK PNPD QEQR NVNL EYQV KINA PKED DYET RITE SKCV TILH KGFS ASVR TILQ NDHS LLAS SWAS AELH APPG SPGT SIVN LTCT TNTT EDNY SRLR SYQV SLHC TWLV GTDA PEDT QYFL YYRY GSWT EECQ EYSK DTLG RNIA CWFP RTFI LSKG RDWL AVLV NGSS KHSA IRPF DQLF ALHA IDQI NPPL NVTA EIEG TRLS IQWE KPVS AFPI HCFD YEVK IHNT RNGY LQIE KLMT NAFI SIID DLSK YDVQ VRAA VSSM CREA GLWS EWSQ PIYV GFSR
Gene ID:3568
Uniprot:Q01344
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:23kDa/37kDa/42kDa/47kDa
Observed MW:76kDa
Synonyms:IL5RA, CD125, CDw125, HSIL5R3, IL5R
Background:The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported.
UniProt Protein Function:IL5RA: This is the receptor for interleukin-5. The alpha chain binds to IL5. Belongs to the type I cytokine receptor family. Type 5 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 3p26-p24

Cellular Component: extracellular space; plasma membrane

Molecular Function:interleukin-5 receptor activity; protein binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: cell proliferation; MAPKKK cascade; signal transduction

NCBI Summary:The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported. [provided by RefSeq, Jul 2011]
UniProt Code:Q01344
NCBI GenInfo Identifier:116242525
NCBI Gene ID:3568
NCBI Accession:Q01344.2
UniProt Secondary Accession:Q01344,Q14633, Q15469, Q6ISX9, B3IU77, B4E2G0,
UniProt Related Accession:Q01344
Molecular Weight:23,737 Da
NCBI Full Name:Interleukin-5 receptor subunit alpha
NCBI Synonym Full Names:interleukin 5 receptor subunit alpha
NCBI Official Symbol:IL5RA  
NCBI Official Synonym Symbols:IL5R; CD125; CDw125; HSIL5R3  
NCBI Protein Information:interleukin-5 receptor subunit alpha
UniProt Protein Name:Interleukin-5 receptor subunit alpha
UniProt Synonym Protein Names:CDw125; CD_antigen: CD125
Protein Family:Interleukin-5 receptor
UniProt Gene Name:IL5RA  
UniProt Entry Name:IL5RA_HUMAN
Anti-IL5RA Polyclonal Antibody (CAB8552)Western blot analysis of extracts of various cell lines, using IL5RA antibody (CAB8552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

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