Anti-IL-20 Antibody (CAB6629)

Antibody Name:	IL20 Rabbit Polyclonal Antibody
Antibody SKU:	CAB6629
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-176 of human IL20 (NP_061194.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-176 of human IL20 (NP_061194.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LKTL NLGS CVIA TNLQ EIRN GFSE IRGS VQAK DGNI DIRI LRRT ESLQ DTKP ANRC CLLR HLLR LYLD RVFK NYQT PDHY TLRK ISSL ANSF LTIK KDLR LCHA HMTC HCGE EAMK KYSQ ILSH FEKL EPQA AVVK ALGE LDIL LQWM EETE
Gene ID:	50604
Uniprot:	Q9NYY1
Cellular Location:	Secreted
Calculated MW:	17kDa/20kDa
Observed MW:	20kDa

Synonyms:

IL20, IL-20, IL10D, ZCYTO10

Background:

The protein encoded by this gene is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis.

UniProt Protein Function:	IL20: Cytokine that may be involved in epidermal function and psoriasis. Acts through STAT3. Belongs to the IL-10 family.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Cytokine; Secreted Chromosomal Location of Human Ortholog: 1q32 Cellular Component: extracellular space; extracellular region Molecular Function:interleukin-20 receptor binding; cytokine activity; interleukin-22 receptor binding Biological Process: positive regulation of osteoclast differentiation; regulation of inflammatory response; positive regulation of epidermal cell differentiation; positive regulation of JAK-STAT cascade; immune response; inflammatory response; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of keratinocyte differentiation
NCBI Summary:	The protein encoded by this gene is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NYY1
NCBI GenInfo Identifier:	38258955
NCBI Gene ID:	50604
NCBI Accession:	Q9NYY1.2
UniProt Secondary Accession:	Q9NYY1,Q17RB3, Q2THG6, Q96QZ6,
UniProt Related Accession:	Q9NYY1
Molecular Weight:	176
NCBI Full Name:	Interleukin-20
NCBI Synonym Full Names:	interleukin 20
NCBI Official Symbol:	IL20
NCBI Official Synonym Symbols:	IL-20; IL10D; ZCYTO10
NCBI Protein Information:	interleukin-20; cytokine Zcyto10; four alpha helix cytokine
UniProt Protein Name:	Interleukin-20
UniProt Synonym Protein Names:	Cytokine Zcyto10
Protein Family:	Interleukin
UniProt Gene Name:	IL20
UniProt Entry Name:	IL20_HUMAN

	Western blot analysis of extracts of mouse liver, using IL20 Antibody (CAB6629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver using IL20 antibody (CAB6629) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using IL20 antibody (CAB6629) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using IL20 antibody (CAB6629) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using IL20 antibody (CAB6629). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
IL20 Antibody (PACO02951)	Human IL-20 CLIA Kit (HUES00207)
	Human IL-20 ELISA Kit (HUES01514)
	Human IL-20 ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)