Anti-IL-24 Antibody (CAB1879)

Antibody Name:	IL24 Rabbit Polyclonal Antibody
Antibody SKU:	CAB1879
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 52-140 of human IL24 (NP_001172085.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse lung, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 52-140 of human IL24 (NP_001172085.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GQEF HFGP CQVK GVVP QKLW EAFW AVKD TMQA QDNI TSAR LLQQ EVLQ NVSD AESC YLVH TLLE FYLK TVFK NYHN RTVE VRTL KSFS T
Gene ID:	11009
Uniprot:	Q13007
Cellular Location:	Secreted
Calculated MW:	7kDa/17kDa/23kDa
Observed MW:	26kDa

Synonyms:

IL24, C49A, FISP, IL10B, MDA7, MOB5, ST16

Background:

This gene encodes a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. The protein encoded by this gene can induce apoptosis selectively in various cancer cells. Overexpression of this gene leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

UniProt Protein Function:	IL24: Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation. Belongs to the IL-10 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 1q32 Cellular Component: extracellular space; endoplasmic reticulum Molecular Function:cytokine activity Biological Process: negative regulation of cell proliferation; wound healing; apoptosis; positive regulation of cell proliferation; positive regulation of JAK-STAT cascade; positive regulation of caspase activity; immune response; negative regulation of caspase activity; inflammatory response; negative regulation of cell migration; positive regulation of tyrosine phosphorylation of Stat3 protein; serine phosphorylation of STAT3 protein
NCBI Summary:	This gene encodes a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. The protein encoded by this gene can induce apoptosis selectively in various cancer cells. Overexpression of this gene leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13007
NCBI GenInfo Identifier:	2497340
NCBI Gene ID:	11009
NCBI Accession:	Q13007.1
UniProt Related Accession:	Q13007
Molecular Weight:
NCBI Full Name:	Interleukin-24
NCBI Synonym Full Names:	interleukin 24
NCBI Official Symbol:	IL24
NCBI Official Synonym Symbols:	C49A; FISP; MDA7; MOB5; ST16; IL10B
NCBI Protein Information:	interleukin-24
UniProt Protein Name:	Interleukin-24
UniProt Synonym Protein Names:	Melanoma differentiation-associated gene 7 protein; MDA-7; Suppression of tumorigenicity 16 protein
Protein Family:	Interleukin
UniProt Gene Name:	IL24
UniProt Entry Name:	IL24_HUMAN

	Western blot analysis of extracts of various cell lines, using IL24 antibody (CAB1879) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse spleen using IL24 Rabbit pAb (CAB1879) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
IL24 Antibody (PACO02096)	Human IL-24 CLIA Kit (HUES01266)
IL24 Antibody (PACO09931)	Human IL-24 ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)