Anti-KLK11 Antibody (CAB6641)

Antibody Name:	Anti-KLK11 Antibody
Antibody SKU:	CAB6641
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human KLK11 (NP_001129504.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	A375, BT-474, HeLa, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human KLK11 (NP_001129504.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MRIL QLIL LALA TGLV GGET RIIK GFEC KPHS QPWQ AALF EKTR LLCG ATLI APRW LLTA AHCL KPRY IVHL GQHN LQKE EGCE QTRT ATES FPHP GFNN SLPN KDHR NDIM LVKM ASPV SITW AVRP LTLS SRCV TAGT SCLI SGWG STSS PQLR LPHT LRCA NITI IEHQ KCEN AYPG NITD TMVC ASVQ EGGK DSCQ GDSG GPLV CNQS LQGI ISWG QDPC AITR KPGV YTKV CKYV DWIQ ETMK NN
Gene ID:	11012
Uniprot:	Q9UBX7
Cellular Location:	Golgi apparatus, Secreted
Calculated MW:	27kDa/30kDa/31kDa/33kDa
Observed MW:	50kDa

Synonyms:

KLK11, PRSS20, TLSP

Background:

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing and the use of alternate promoters results in multiple transcript variants encoding distinct isoforms which are differentially expressed.

UniProt Protein Function:	KLK11: Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. Belongs to the peptidase S1 family. Kallikrein subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.4.21.-; Protease; Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 19q13.41 Cellular Component: extracellular space Molecular Function:serine-type peptidase activity
NCBI Summary:	Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing and the use of alternate promoters results in multiple transcript variants encoding distinct isoforms which are differentially expressed. [provided by RefSeq, Dec 2016]
UniProt Code:	Q9UBX7
NCBI GenInfo Identifier:	88984315
NCBI Gene ID:	11012
NCBI Accession:	Q9UBX7.2
UniProt Secondary Accession:	Q9UBX7,O75837, Q0WXX5, Q8IXD7, Q9NS65,
UniProt Related Accession:	Q9UBX7
Molecular Weight:	31kDa
NCBI Full Name:	Kallikrein-11
NCBI Synonym Full Names:	kallikrein related peptidase 11
NCBI Official Symbol:	KLK11
NCBI Official Synonym Symbols:	TLSP; PRSS20
NCBI Protein Information:	kallikrein-11
UniProt Protein Name:	Kallikrein-11
UniProt Synonym Protein Names:	Hippostasin; Serine protease 20; Trypsin-like protease
Protein Family:	Kallikrein
UniProt Gene Name:	KLK11

	Western blot analysis of extracts of various cell lines, using KLK11 Antibody (CAB6641) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 20s.
	Immunohistochemistry of paraffin-embedded mouse brain using KLK11 antibody (CAB6641) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using KLK11 antibody (CAB6641) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-KLK11 Antibody (CAB12565)	HumanÂ KLK11Â (KallikreinÂ 11)Â ELISAÂ Kit (HUFI03772)
Cleaved-KLK11 (I54) Antibody (PACO00030)	Human Kallikrein-11 (KLK11) ELISA Kit
KLK11 Antibody (PACO10143)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)