Anti-KATNA1 Antibody (CAB16491)

Antibody Name:	Anti-KATNA1 Antibody
Antibody SKU:	CAB16491
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-167 of human KATNA1 (NP_001191005.1).

Application:	WB IHC
Recommended Dilution:	WB 1:1000 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-167 of human KATNA1 (NP_001191005.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSLL MISE NVKL AREY ALLG NYDS AMVY YQGV LDQM NKYL YSVK DTYL QQKW QQVW QEIN VEAK HVKD IMKT LESF KLDS TPLK AAQH DLPA SEGE VWSM PVPV ERRP SPGP RKRQ SSQY SDPK SHGN RPST TVRV HRSS AQNV HNDR GKAV RCRE KKEQ NKGR EEK
Gene ID:	11104
Uniprot:	O75449
Cellular Location:	Cytoplasm, Midbody, centrosome, cytoskeleton, microtubule organizing center, spindle pole
Calculated MW:	35kDa/55kDa
Observed MW:

Synonyms:

KATNA1

Background:

Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help to organize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60 subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemble microtubules, while the p80 subunit targets the enzyme to the centrosome. This gene encodes the p80 subunit. This protein is a member of the AAA family of ATPases. Multiple alternatively spliced variants, encoding the same protein, have been identified.

UniProt Protein Function:	KATNA1: Severs microtubules in vitro in an ATP-dependent manner. This activity may promote rapid reorganization of cellular microtubule arrays, such as during disassembly of interphase microtubules at the G2-M transition. May also be required for microtubule release from the centrosome after nucleation. In mitotic spindles this could allow depolymerization of the microtubule end proximal to the centrosome, and subsequent poleward microtubule flux. In neurons, microtubule release within the cell body may allow their subsequent transport into neuronal processes by microtubule dependent motor proteins. This transport is required for axonal growth. Belongs to the AAA ATPase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Hydrolase; Motility/polarity/chemotaxis; EC 3.6.4.3 Chromosomal Location of Human Ortholog: 6q25.1 Cellular Component: cytoplasm; microtubule organizing center; midbody; nucleus; spindle; spindle pole Molecular Function:microtubule binding; microtubule-severing ATPase activity; protein binding; protein heterodimerization activity Biological Process: cytoplasmic microtubule organization and biogenesis
NCBI Summary:	Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help to organize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60 subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemble microtubules, while the p80 subunit targets the enzyme to the centrosome. This gene encodes the p80 subunit. This protein is a member of the AAA family of ATPases. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2011]
UniProt Code:	O75449
NCBI GenInfo Identifier:	60390161
NCBI Gene ID:	11104
NCBI Accession:	O75449.1
UniProt Secondary Accession:	O75449,Q5TFA8, Q5TFA9, Q86VN2, Q9NU52, E1P5A3,
UniProt Related Accession:	O75449
Molecular Weight:	35,319 Da
NCBI Full Name:	Katanin p60 ATPase-containing subunit A1
NCBI Synonym Full Names:	katanin catalytic subunit A1
NCBI Official Symbol:	KATNA1
NCBI Protein Information:	katanin p60 ATPase-containing subunit A1
UniProt Protein Name:	Katanin p60 ATPase-containing subunit A1
UniProt Synonym Protein Names:	p60 katanin
UniProt Gene Name:	KATNA1
UniProt Entry Name:	KTNA1_HUMAN

	Immunohistochemistry of paraffin-embedded rat heart using KATNA1 antibody (CAB16491) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human tonsil using KATNA1 antibody (CAB16491) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using KATNA1 antibody (CAB16491) at dilution of 1:100 (40x lens).

Antibodies

KATNA1 Antibody (PACO10041)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)