Anti-LARP1B Antibody (CAB13222)

Antibody Name:	Anti-LARP1B Antibody
Antibody SKU:	CAB13222
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 170-320 of human LARP1B (NP_060548.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, A-431

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 170-320 of human LARP1B (NP_060548.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FDYS YGYQ EHGE RTDQ PFQT ELNT SMMY YYDD GTGV QVYP VEEA LLKE YIKR QIEY YFSV ENLE RDFF LRGK MDEQ GFLP ISLI AGFQ RVQA LTTN LNLI LEAL KDST EVEI VDEK MRKK IEPE KWPI PGPP PRSV PPTD FSQL IDCP EFV
Gene ID:	55132
Uniprot:	Q659C4
Cellular Location:
Calculated MW:	14kDa/24-38kDa/90-105kDa
Observed MW:	105KDa

Synonyms:

LARP1B, LARP2

Background:

This gene encodes a protein containing domains found in the La related protein of Drosophila melanogaster. La motif-containing proteins are thought to be RNA-binding proteins, where the La motif and adjacent amino acids fold into an RNA recognition motif. The La motif is also found in proteins unrelated to the La protein. Alternative splicing has been observed at this locus and multiple variants, encoding distinct isoforms, are described. Additional splice variation has been identified but the full-length nature of these transcripts has not been determined.

UniProt Protein Function:	LARP2: a protein containing domains found in the La related protein of Drosophila melanogaster. La motif-containing proteins are thought to be RNA-binding proteins, where the La motif and adjacent amino acids fold into an RNA recognition motif. The La motif is also found in proteins unrelated to the La protein. Alternative splicing has been observed at this locus and three variants, encoding distinct isoforms, are described. Additional splice variation has been identified but the full-length nature of these transcripts has not been determined. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Unknown function Chromosomal Location of Human Ortholog: 4q28.2 Cellular Component: nucleus Molecular Function:protein binding
NCBI Summary:	This gene encodes a protein containing domains found in the La related protein of Drosophila melanogaster. La motif-containing proteins are thought to be RNA-binding proteins, where the La motif and adjacent amino acids fold into an RNA recognition motif. The La motif is also found in proteins unrelated to the La protein. Alternative splicing has been observed at this locus and multiple variants, encoding distinct isoforms, are described. Additional splice variation has been identified but the full-length nature of these transcripts has not been determined. [provided by RefSeq, Jun 2013]
UniProt Code:	Q659C4
NCBI GenInfo Identifier:	158564329
NCBI Gene ID:	55132
NCBI Accession:	Q659C4.2
UniProt Secondary Accession:	Q659C4,Q2YDB6, Q4W599, Q4W5B2, Q659A0, Q6P5X2, Q7Z3F7 Q86VK7, Q8N6F4, Q8N7H4, Q8NAF2, Q9H5E7,
UniProt Related Accession:	Q659C4
Molecular Weight:	90,405 Da
NCBI Full Name:	La-related protein 1B
NCBI Synonym Full Names:	La ribonucleoprotein domain family member 1B
NCBI Official Symbol:	LARP1B
NCBI Official Synonym Symbols:	LARP2
NCBI Protein Information:	la-related protein 1B
UniProt Protein Name:	La-related protein 1B
UniProt Synonym Protein Names:	La ribonucleoprotein domain family member 1B; La ribonucleoprotein domain family member 2; La-related protein 2
Protein Family:	La-related protein
UniProt Gene Name:	LARP1B
UniProt Entry Name:	LAR1B_HUMAN

	Western blot analysis of extracts of various cell lines, using LARP1B Rabbit pAb (CAB13222) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded Mouse testis using LARP1B Rabbit pAb (CAB13222) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat ovary using LARP1B Rabbit pAb (CAB13222) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human breast cancer using LARP1B Rabbit pAb (CAB13222) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)