Anti-MLH3 Antibody (CAB7796)

Antibody Name:	Anti-MLH3 Antibody
Antibody SKU:	CAB7796
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1180-1429 of human MLH3 (NP_055196.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	MCF7, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1180-1429 of human MLH3 (NP_055196.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RFTK GMIH SMQV LQQV DNKF IACL MSTK TEEN GEAD SYEK QQAQ GSGR KKLL SSTL IPPL EITV TEEQ RRLL WCYH KNLE DLGL EFVF PDTS DSLV LVGK VPLC FVER EANE LRRG RSTV TKSI VEEF IREQ LELL QTTG GIQG TLPL TVQK VLAS QACH GAIK FNDG LSLQ ESCR LIEA LSSC QLPF QCAH GRPS MLPL ADID HLEQ EKQI KPNL TKLR KMAQ AWRL FGKA ECDT RQSL QQSM PPCE PP
Gene ID:	27030
Uniprot:	Q9UHC1
Cellular Location:	Nucleus
Calculated MW:	161kDa/163kDa
Observed MW:	164kDa

Synonyms:

MLH3, HNPCC7

Background:

This gene is a member of the MutL-homolog (MLH) family of DNA mismatch repair (MMR) genes. MLH genes are implicated in maintaining genomic integrity during DNA replication and after meiotic recombination. The protein encoded by this gene functions as a heterodimer with other family members. Somatic mutations in this gene frequently occur in tumors exhibiting microsatellite instability, and germline mutations have been linked to hereditary nonpolyposis colorectal cancer type 7 (HNPCC7). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.

UniProt Protein Function:	Probably involved in the repair of mismatches in DNA.
NCBI Summary:	This gene is a member of the MutL-homolog (MLH) family of DNA mismatch repair (MMR) genes. MLH genes are implicated in maintaining genomic integrity during DNA replication and after meiotic recombination. The protein encoded by this gene functions as a heterodimer with other family members. Somatic mutations in this gene frequently occur in tumors exhibiting microsatellite instability, and germline mutations have been linked to hereditary nonpolyposis colorectal cancer type 7 (HNPCC7). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UHC1
NCBI GenInfo Identifier:	91992162
NCBI Gene ID:	27030
NCBI Accession:	NP_001035197.1
UniProt Secondary Accession:	Q9UHC1,P49751, Q56DK9, Q9P292, Q9UHC0,
UniProt Related Accession:	Q9UHC1
Molecular Weight:	164 kD
NCBI Full Name:	DNA mismatch repair protein Mlh3 isoform 1
NCBI Synonym Full Names:	mutL homolog 3
NCBI Official Symbol:	MLH3
NCBI Official Synonym Symbols:	HNPCC7
NCBI Protein Information:	DNA mismatch repair protein Mlh3
UniProt Protein Name:	DNA mismatch repair protein Mlh3
UniProt Synonym Protein Names:	MutL protein homolog 3
Protein Family:	DNA mismatch repair protein
UniProt Gene Name:	MLH3

	Western blot analysis of extracts of various cell lines, using MLH3 antibody (CAB7796) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using MLH3 antibody (CAB7796) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

MLH3 Antibody (PACO03873)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)