The NFAT5 Antibody (CAB16649) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blotting applications.NFAT5, also known as TonEBP, plays a crucial role in regulating cellular responses to changes in osmolarity and stress, as well as mediating inflammatory processes. Its involvement in immune cell function and the pathogenesis of diseases such as cancer and autoimmune disorders makes it an important target for drug development and therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
NFAT5 Antibody
SKU:
CAB16649
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NFATZ, OREBP, NF-AT5, NFATL1, TONEBP, NFAT5
Positive Sample:
SH-SY5Y, A-549
Cellular Localization:
Cytosol, Nucleoplasm, Nucleus.
Calculated MW:
166kDa
Observed MW:
174kDa
The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
10725
RRID
AB_2770572
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NFAT5 Rabbit pAb (CAB16649) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
