Anti-OAZ1 Antibody (CAB7444)

Antibody Name:	Anti-OAZ1 Antibody
Antibody SKU:	CAB7444
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-68 of human OAZ1 (NP_004143.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, Mouse heart, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-68 of human OAZ1 (NP_004143.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVKS SLQR ILNS HCFA REKE GDKP SATI HASR TMPL LSLH SRGG SSSE SSRV SLHC CSNP GPGP RWCS
Gene ID:	4946
Uniprot:	P54368
Cellular Location:
Calculated MW:	25kDa
Observed MW:	27kDa

Synonyms:

OAZ1, AZ1, AZI, OAZ

Background:

The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamines. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis; thus, completing the auto-regulatory circuit. This gene encodes antizyme 1, the first member of the antizyme family, that has broad tissue distribution, and negatively regulates intracellular polyamine levels by binding to and targeting ODC for degradation, as well as inhibiting polyamine uptake. Antizyme 1 mRNA contains two potential in-frame AUGs; and studies in rat suggest that alternative use of the two translation initiation sites results in N-terminally distinct protein isoforms with different subcellular localization. Alternatively spliced transcript variants have also been noted for this gene.

UniProt Protein Function:	OAZ1: Binds to, and destabilizes, ornithine decarboxylase which is then degraded. Also inhibits cellular uptake of polyamines by inactivating the polyamine uptake transporter. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. Belongs to the ODC antizyme family. 1 isoforms of the human protein are produced by ribosomal frameshifting.
UniProt Protein Details:	Protein type:Inhibitor Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: cytosol Molecular Function:protein binding; ornithine decarboxylase inhibitor activity Biological Process: transport; polyamine biosynthetic process; negative regulation of catalytic activity; positive regulation of protein catabolic process; regulation of amino acid metabolic process
NCBI Summary:	The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamines. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis; thus, completing the auto-regulatory circuit. This gene encodes antizyme 1, the first member of the antizyme family, that has broad tissue distribution, and negatively regulates intracellular polyamine levels by binding to and targeting ODC for degradation, as well as inhibiting polyamine uptake. Antizyme 1 mRNA contains two potential in-frame AUGs; and studies in rat suggest that alternative use of the two translation initiation sites results in N-terminally distinct protein isoforms with different subcellular localization. Alternatively spliced transcript variants have also been noted for this gene. [provided by RefSeq, Dec 2014]
UniProt Code:	P54368
NCBI GenInfo Identifier:	1709427
NCBI Gene ID:	4946
NCBI Accession:	P54368.3
UniProt Secondary Accession:	P54368,O43382, Q14989, Q92595, Q9UPL9,
UniProt Related Accession:	P54368
Molecular Weight:	228
NCBI Full Name:	Ornithine decarboxylase antizyme 1
NCBI Synonym Full Names:	ornithine decarboxylase antizyme 1
NCBI Official Symbol:	OAZ1
NCBI Official Synonym Symbols:	AZI; OAZ
NCBI Protein Information:	ornithine decarboxylase antizyme 1; ODC-Az
UniProt Protein Name:	Ornithine decarboxylase antizyme 1
Protein Family:	Ornithine decarboxylase antizyme
UniProt Gene Name:	OAZ1
UniProt Entry Name:	OAZ1_HUMAN

	Western blot analysis of extracts of various cell lines, using OAZ1 antibody (CAB7444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat kidney using OAZ1 antibody (CAB7444) at dilution of 1:100 (40x lens).

Antibodies

OAZ1 Antibody (PACO04479)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)