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Anti-PADI4 Antibody (CAB1906)

SKU:
CAB1906
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
  • Immunology Antibodies 2 Anti-PADI4 Antibody CAB1906
  • Immunology Antibodies 2 Anti-PADI4 Antibody CAB1906
  • Immunology Antibodies 2 Anti-PADI4 Antibody CAB1906
€139 - €419
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Description

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Antibody Name:Anti-PADI4 Antibody
Antibody SKU:CAB1906
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PADI4 (NP_036519.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50
Reactivity:Human, Mouse, Rat
Positive Samples:BT-474, SKOV3
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PADI4 (NP_036519.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAQG TLIR VTPE QPTH AVCV LGTL TQLD ICSS APED CTSF SINA SPGV VVDI AHGP PAKK KSTG SSTW PLDP GVEV TLTM KVAS GSTG DQKV QISY YGPK TPPV KALL YLTG VEIS LCAD ITRT GKVK PTRA VKDQ RTWT WGPC GQGA ILLV NCDR DNLE SSAM DCED DEVL DSED LQDM SLMT LSTK TPKD FFTN HTLV LHVA RSEM DKVR VFQA TRGK LSSK CSVV LGPK WPSH YLMV PGGK HNMD FY
Gene ID:23569
Uniprot:Q9UM07
Cellular Location:Cytoplasm, Cytoplasmic granule, Nucleus
Calculated MW:74kDa
Observed MW:74kDa
Synonyms:PADI4, PAD, PAD4, PADI5, PDI4, PDI5
Background:This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response.
UniProt Protein Function:PADI4: Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1. Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA). It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Belongs to the protein arginine deiminase family.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; Hydrolase; EC 3.5.3.15

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: cytoplasm; nucleus

Molecular Function:arginine deiminase activity; protein binding; calcium ion binding; protein-arginine deiminase activity

Biological Process: chromatin remodeling; nucleosome assembly; transcription, DNA-dependent; regulation of transcription, DNA-dependent; stem cell maintenance; innate immune response; protein modification process; chromatin modification; peptidyl-citrulline biosynthetic process from peptidyl-arginine

Disease: Rheumatoid Arthritis

NCBI Summary:This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq, Jul 2008]
UniProt Code:Q9UM07
NCBI GenInfo Identifier:296439260
NCBI Gene ID:23569
NCBI Accession:Q9UM07.2
UniProt Related Accession:Q9UM07
Molecular Weight:
NCBI Full Name:Protein-arginine deiminase type-4
NCBI Synonym Full Names:peptidyl arginine deiminase 4
NCBI Official Symbol:PADI4  
NCBI Official Synonym Symbols:PAD; PAD4; PDI4; PDI5; PADI5  
NCBI Protein Information:protein-arginine deiminase type-4
UniProt Protein Name:Protein-arginine deiminase type-4
UniProt Synonym Protein Names:HL-60 PAD; Peptidylarginine deiminase IV; Protein-arginine deiminase type IV
Protein Family:Lipase
UniProt Gene Name:PADI4  
UniProt Entry Name:PADI4_HUMAN
Anti-PADI4 Antibody (CAB1906)Western blot analysis of extracts of various cell lines, using PADI4 antibody (CAB1906) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-PADI4 Antibody (CAB1906)Immunohistochemistry of paraffin-embedded rat lung using PADI4 antibody (CAB1906) at dilution of 1:100 (40x lens).
Anti-PADI4 Antibody (CAB1906)Immunofluorescence analysis of U2OS cells using PADI4 antibody (CAB1906). Blue: DAPI for nuclear staining.

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