Anti-PALLD Antibody (CAB12362)

Antibody Name:	Anti-PALLD Antibody
Antibody SKU:	CAB12362
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 383-672 of human PALLD (NP_001159582.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, A-549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 383-672 of human PALLD (NP_001159582.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AVNQ RGRS PRSP SGHP HVRR PRSR SRDS GDEN EPIQ ERFF RPHF LQAP GDLT VQEG KLCR MDCK VSGL PTPD LSWQ LDGK PVRP DSAH KMLV RENG VHSL IIEP VTSR DAGI YTCI ATNR AGQN SFSL ELVV AAKE AHKP PVFI EKLQ NTGV ADGY PVRL ECRV LGVP PPQI FWKK ENES LTHS TDRV SMHQ DNHG YICL LIQG ATKE DAGW YTVS AKNE AGIV SCTA RLDV YTQW HQQS QSTK PKKV RPSA SRYA ALSD QGLD IKAA FQPE ANPS HLTL NTAL VESE DL
Gene ID:	23022
Uniprot:	Q8WX93
Cellular Location:	Cell junction, Cell projection, Cytoplasm, Z line, cytoskeleton, focal adhesion, lamellipodium, myofibril, ruffle, sarcomere
Calculated MW:	42kDa/58kDa/73kDa/85-150kDa
Observed MW:	150kDa

Synonyms:

PALLD, CGI-151, CGI151, MYN, PNCA1, SIH002, palladin

Background:

This gene encodes a cytoskeletal protein that is required for organizing the actin cytoskeleton. The protein is a component of actin-containing microfilaments, and it is involved in the control of cell shape, adhesion, and contraction. Polymorphisms in this gene are associated with a susceptibility to pancreatic cancer type 1, and also with a risk for myocardial infarction. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	palladin: Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific subcellular foci. Genetic variations in PALLD are associated with susceptibility to pancreatic cancer type 1 (PNCA1). Expression is increased early in the development of pancreatic cancer: in normal-appearing whole tissue immediately adjacent to cancer, in the pre-cancer, and in both the familial and sporadic forms of the cancer. Genetic variations in PALLD may be associated with myocardial infarction. Belongs to the myotilin/palladin family. 9 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 4q32.3 Cellular Component: ruffle; focal adhesion; mitochondrion; lamellipodium; cytoplasm; plasma membrane; actin filament; nucleus; Z disc; actin cytoskeleton Molecular Function:protein binding; actin binding; muscle alpha-actinin binding Biological Process: cytoskeleton organization and biogenesis Disease: Pancreatic Cancer, Susceptibility To, 1
NCBI Summary:	This gene encodes a cytoskeletal protein that is required for organizing the actin cytoskeleton. The protein is a component of actin-containing microfilaments, and it is involved in the control of cell shape, adhesion, and contraction. Polymorphisms in this gene are associated with a susceptibility to pancreatic cancer type 1, and also with a risk for myocardial infarction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
UniProt Code:	Q8WX93
NCBI GenInfo Identifier:	313104206
NCBI Gene ID:	23022
NCBI Accession:	Q8WX93.3
UniProt Secondary Accession:	Q8WX93,Q7L3E0, Q7Z3W0, Q86WE8, Q8N1M2, Q9UGA0, Q9UQF5 Q9Y2J6, Q9Y3E9, B3KTG2, B5MD56, B7ZMM5,
UniProt Related Accession:	Q8WX93
Molecular Weight:	1383
NCBI Full Name:	Palladin
NCBI Synonym Full Names:	palladin, cytoskeletal associated protein
NCBI Official Symbol:	PALLD
NCBI Official Synonym Symbols:	MYN; PNCA1; CGI151; SIH002; CGI-151
NCBI Protein Information:	palladin; myoneurin; sarcoma antigen NY-SAR-77
UniProt Protein Name:	Palladin
UniProt Synonym Protein Names:	SIH002; Sarcoma antigen NY-SAR-77
Protein Family:	Palladin
UniProt Gene Name:	PALLD
UniProt Entry Name:	PALLD_HUMAN

	Western blot analysis of extracts of various cell lines, using PALLD antibody (CAB12362) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat ovary using PALLD Rabbit pAb (CAB12362) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using PALLD Rabbit pAb (CAB12362) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using PALLD Rabbit pAb (CAB12362) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using PALLD Rabbit pAb (CAB12362) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-PALLD Antibody (CAB5529)

PALLD Antibody (PACO05893)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)