Anti-PIAS3 Antibody (CAB7060)

Antibody Name:	Anti-PIAS3 Antibody
Antibody SKU:	CAB7060
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 452-628 of human PIAS3 (NP_006090.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Rat kidney, Rat brain, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 452-628 of human PIAS3 (NP_006090.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IESS SDEE DLPP TKKH CSVT SAAI PALP GSKG VLTS GHQP SSVL RSPA MGTL GGDF LSSL PLHE YPPA FPLG ADIQ GLDL FSFL QTES QHYG PSVI TSLD EQDA LGHF FQYR GTPS HFLG PLAP TLGS SHCS ATPA PPPG RVSS IVAP GGAL REGH GGPL PSGP SLTG CRSD IISL D
Gene ID:	10401
Uniprot:	Q9Y6X2
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	68kDa
Observed MW:	68kDa

Synonyms:

PIAS3, ZMIZ5

Background:

This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined.

UniProt Protein Function:	PIAS3: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Monomer. Binds SUMO1 and UBE2I. Interacts with BCL11A, HMGA2, IRF1, MITF and NCOA2. Interacts with STAT5; the interaction occurs on stimulation by PRL. Interacts with GFI1; the interaction relieves the inhibitory effect of PIAS3 on STAT3- mediated transcriptional activity. Interacts with AR, PLAG1 and ZFHX3. Interacts with STAT3; the interaction occurs on stimulation by IL6, CNTF or OSM and inhibits the DNA binding activity of STAT3. By dihydrotestosterone (DHT) in prostate cancer cells. Isoform 1 is expressed in most tissues except thymus and small intestine. Isoform 3 is expressed only in brain, heart, thymus, muscle, lung, testis, lactating breast and embryonic stem cells. Belongs to the PIAS family.
UniProt Protein Details:	Protein type:EC 6.3.2.-; SUMO conjugating system; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 1q21 Cellular Component: cytoplasm; dendrite; synapse; nucleus Molecular Function:protein C-terminus binding; protein binding; potassium channel regulator activity; enzyme binding; zinc ion binding; protein N-terminus binding; SUMO ligase activity; ligase activity Biological Process: positive regulation of protein sumoylation; protein sumoylation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; response to hormone stimulus; positive regulation of membrane potential
NCBI Summary:	This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y6X2
NCBI GenInfo Identifier:	56405390
NCBI Gene ID:	10401
NCBI Accession:	Q9Y6X2.2
UniProt Secondary Accession:	Q9Y6X2,Q9UFI3,
UniProt Related Accession:	Q9Y6X2
Molecular Weight:	628
NCBI Full Name:	E3 SUMO-protein ligase PIAS3
NCBI Synonym Full Names:	protein inhibitor of activated STAT, 3
NCBI Official Symbol:	PIAS3
NCBI Official Synonym Symbols:	ZMIZ5
NCBI Protein Information:	E3 SUMO-protein ligase PIAS3; zinc finger, MIZ-type containing 5; protein inhibitor of activated STAT protein 3
UniProt Protein Name:	E3 SUMO-protein ligase PIAS3
UniProt Synonym Protein Names:	Protein inhibitor of activated STAT protein 3
Protein Family:	E3 SUMO-protein ligase
UniProt Gene Name:	PIAS3
UniProt Entry Name:	PIAS3_HUMAN

	Western blot analysis of extracts of various cell lines, using PIAS3 antibody (CAB7060) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using PIAS3 antibody (CAB7060) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using PIAS3 antibody (CAB7060) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-PIAS3 Antibody (CAB13394)	Human PIAS3 (Protein Inhibitor of Activated STAT3) ELISA Kit (HUES02061)
PIAS3 Antibody (PACO06750)	Human E3 SUMO-protein ligase PIAS3 (PIAS3) ELISA Kit
PIAS3 Antibody (PACO11232)	PIAS3 Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)