Anti-PPAN Antibody (CAB7377)

Antibody Name:	Anti-PPAN Antibody
Antibody SKU:	CAB7377
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PPAN (NP_064615.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	U-87MG, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PPAN (NP_064615.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGQS GRSR HQKR ARAQ AQLR NLEA YAAN PHSF VFTR GCTG RNIR QLSL DVRR VMEP LTAS RLQV RKKN SLKD CVAV AGPL GVTH FLIL SKTE TNVY FKLM RLPG GPTL TFQV KKYS LVRD VVSS LRRH RMHE QQFA HPPL LVLN SFGP HGMH VKLM ATMF QNLF PSIN VHKV NLNT IKRC LLID YNPD SQEL DFRH YSIK VVPV GASR GMKK LLQE KFPN MSRL QDIS ELLA TGAG LSES EAEP DGDH NITE LPQA VAGR GNMR AQQS AVRL TEIG PRMT LQLI KVQE GVGE GKVM FHSF
Gene ID:	56342
Uniprot:	Q9NQ55
Cellular Location:	Nucleus, nucleolus
Calculated MW:	51kDa/53kDa/58kDa
Observed MW:	53kDa/55kDa

Synonyms:

PPAN, BXDC3, SSF, SSF-1, SSF1, SSF2

Background:

The protein encoded by this gene is an evolutionarily conserved protein similar to yeast SSF1 as well as to the gene product of the Drosophila gene peter pan (ppan). SSF1 is known to be involved in the second step of mRNA splicing. Both SSF1 and ppan are essential for cell growth and proliferation. Exogenous expression of this gene was reported to reduce the anchorage-independent growth of some tumor cells. Read-through transcription of this gene with P2RY11/P2Y(11), an adjacent downstream gene that encodes an ATP receptor, has been found. These read-through transcripts are ubiquitously present and up-regulated during granulocyte differentiation.

UniProt Protein Function:	SSF1: May have a role in cell growth. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA-binding; Nucleolus Chromosomal Location of Human Ortholog: 19p13 Cellular Component: nucleolus; nucleus Biological Process: RNA splicing
NCBI Summary:	The protein encoded by this gene is an evolutionarily conserved protein similar to yeast SSF1 as well as to the gene product of the Drosophila gene peter pan (ppan). SSF1 is known to be involved in the second step of mRNA splicing. Both SSF1 and ppan are essential for cell growth and proliferation. Exogenous expression of this gene was reported to reduce the anchorage-independent growth of some tumor cells. Read-through transcription of this gene with P2RY11/P2Y(11), an adjacent downstream gene that encodes an ATP receptor, has been found. These read-through transcripts are ubiquitously present and up-regulated during granulocyte differentiation. [provided by RefSeq, Nov 2010]
UniProt Code:	Q9NQ55
NCBI GenInfo Identifier:	21264056
NCBI Gene ID:	56342
NCBI Accession:	Q9NQ55.1
UniProt Secondary Accession:	Q9NQ55,Q9BW97, Q9H170, C9J3F9,
UniProt Related Accession:	Q9NQ55
Molecular Weight:	473
NCBI Full Name:	Suppressor of SWI4 1 homolog
NCBI Synonym Full Names:	peter pan homolog (Drosophila)
NCBI Official Symbol:	PPAN
NCBI Official Synonym Symbols:	SSF; SSF1; SSF2; BXDC3; SSF-1
NCBI Protein Information:	suppressor of SWI4 1 homolog; suppressor of sterile four 1; homolog of S. cerevisiae SSF1; second-step splicing factor 1; brix domain-containing protein 3
UniProt Protein Name:	Suppressor of SWI4 1 homolog
UniProt Synonym Protein Names:	Brix domain-containing protein 3; Peter Pan homolog
Protein Family:	Protein
UniProt Gene Name:	PPAN
UniProt Entry Name:	SSF1_HUMAN

	Western blot analysis of extracts of various cell lines, using PPAN Antibody (CAB7377) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human prostate using PPAN antibody (CAB7377) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using PPAN antibody (CAB7377) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of A549 cells using PPAN antibody (CAB7377).

Antibodies

PPAN Antibody (PACO11383)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)