Anti-PPAT Antibody (CAB6698)

Antibody Name:	Anti-PPAT Antibody
Antibody SKU:	CAB6698
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PPAT (NP_002694.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, COS-7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PPAT (NP_002694.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MELE ELGI REEC GVFG CIAS GEWP TQLD VPHV ITLG LVGL QHRG QESA GIVT SDGS SVPT FKSH KGMG LVNH VFTE DNLK KLYV SNLG IGHT RYAT TGKC ELEN CQPF VVET LHGK IAVA HNGE LVNA ARLR KKLL RHGI GLST SSDS EMIT QLLA YTPP QEQD DTPD WVAR IKNL MKEA PTAY SLLI MHRD VIYA VRDP YGNR PLCI GRLI PVSD INDK EKKT SETE GWVV SSES CSFL SIGA RYYR EVLP GEIV EISR HNVQ TLDI ISRS EGNP VAFC
Gene ID:	5471
Uniprot:	Q06203
Cellular Location:
Calculated MW:	57kDa
Observed MW:	55kDa

Synonyms:

PPAT, ATASE, GPAT, PRAT

Background:

The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region.

UniProt Protein Function:	ATASE: Homotetramer.
UniProt Protein Details:	Protein type:Nucleotide Metabolism - purine; Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase; EC 2.4.2.14 Chromosomal Location of Human Ortholog: 4q12 Cellular Component: cytosol Molecular Function:amidophosphoribosyltransferase activity Biological Process: purine nucleotide biosynthetic process; purine ribonucleoside monophosphate biosynthetic process
NCBI Summary:	The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq, Mar 2011]
UniProt Code:	Q06203
NCBI GenInfo Identifier:	548638
NCBI Gene ID:	5471
NCBI Accession:	Q06203.1
UniProt Related Accession:	Q06203
Molecular Weight:	57,399 Da
NCBI Full Name:	Amidophosphoribosyltransferase
NCBI Synonym Full Names:	phosphoribosyl pyrophosphate amidotransferase
NCBI Official Symbol:	PPAT
NCBI Official Synonym Symbols:	GPAT; PRAT; ATASE
NCBI Protein Information:	amidophosphoribosyltransferase
UniProt Protein Name:	Amidophosphoribosyltransferase
UniProt Synonym Protein Names:	Glutamine phosphoribosylpyrophosphate amidotransferase; GPAT
Protein Family:	Pyridoxamine--pyruvate transaminase
UniProt Gene Name:	PPAT
UniProt Entry Name:	PUR1_HUMAN

	Western blot analysis of extracts of various cell lines, using PPAT antibody (CAB6698) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using PPAT Polyclonal Antibody (CAB6698) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

PPAT Antibody (PACO11388)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)