Anti-SSX2 Antibody (CAB6989)

Antibody Name:	Anti-SSX2 Antibody
Antibody SKU:	CAB6989
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-188 of human SSX2 (NP_783629.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-188 of human SSX2 (NP_783629.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNGD DAFA RRPT VGAQ IPEK IQKA FDDI AKYF SKEE WEKM KASE KIFY VYMK RKYE AMTK LGFK ATLP PFMC NKRA EDFQ GNDL DNDP NRGN QVER PQMT FGRL QGIS PKIM PKKP AEEG NDSE EVPE ASGP QNDG KELC PPGK PTTS EKIH ERSG PKRG EHAW THRL RERK QLVI YEEI SDPE EDDE
Gene ID:	6757
Uniprot:	Q16385
Cellular Location:	Nucleus
Calculated MW:	21kDa/25kDa
Observed MW:	22kDa

Synonyms:

SSX2, CT5.2, CT5.2A, HD21, HOM-MEL-40, SSX

Background:

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneous humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. This gene, and also the SSX1 and SSX4 family members, have been involved in t(X;18)(p11.2;q11.2) translocations that are characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are likely responsible for transforming activity. Alternative splicing of this gene results in multiple transcript variants. This gene also has an identical duplicate, GeneID: 727837, located about 45 kb downstream in the opposite orientation on chromosome X.

UniProt Protein Function:	SSX2B: Could act as a modulator of transcription. A chromosomal aberration involving SSX2 may be a cause of synovial sarcoma. Translocation t(X;18)(p11.2;q11.2). The translocation is specifically found in more than 80% of synovial sarcoma. The fusion products SSXT-SSX1 or SSXT-SSX2 are probably responsible for transforming activity. Heterogeneity in the position of the breakpoint can occur (low frequency). Belongs to the SSX family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cancer Testis Antigen (CTA) Chromosomal Location of Human Ortholog: Xp11.22 Cellular Component: nucleus Molecular Function:protein binding; nucleic acid binding Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent
NCBI Summary:	The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneous humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. This gene, and also the SSX1 and SSX4 family members, have been involved in t(X;18)(p11.2;q11.2) translocations that are characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are likely responsible for transforming activity. Alternative splicing of this gene results in multiple transcript variants. This gene also has an identical duplicate, GeneID: 6757, located about 45 kb upstream in the opposite orientation on chromosome X. [provided by RefSeq, Jul 2013]
UniProt Code:	Q16385
NCBI GenInfo Identifier:	3915028
NCBI Gene ID:	6757
NCBI Accession:	Q16385.2
UniProt Secondary Accession:	Q16385,Q16404, Q5JS26, Q96IP7, Q9BU88,
UniProt Related Accession:	Q16385
Molecular Weight:	21kDa
NCBI Full Name:	Protein SSX2
NCBI Synonym Full Names:	synovial sarcoma, X breakpoint 2B
NCBI Official Symbol:	SSX2B
NCBI Official Synonym Symbols:	SSX; CT5.2; CT5.2b; HOM-MEL-40
NCBI Protein Information:	protein SSX2; tumor antigen HOM-MEL-40; cancer/testis antigen 5.2; cancer/testis antigen family 5, member 2b
UniProt Protein Name:	Protein SSX2
UniProt Synonym Protein Names:	Cancer/testis antigen 5.2; CT5.2; Synovial sarcoma, X breakpoint 2; Tumor antigen HOM-MEL-40
Protein Family:	Protein
UniProt Gene Name:	SSX2
UniProt Entry Name:	SSX2_HUMAN

	Western blot analysis of extracts of HeLa cells, using SSX2 antibody (CAB6989) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using SSX2 antibody (CAB6989) at dilution of 1:200 (40x lens).

Antibodies

Anti-SSX2 Antibody (CAB13548)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)