Anti-RAB6A Antibody (CAB5613)

Antibody Name:	Anti-RAB6A Antibody
Antibody SKU:	CAB5613
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human RAB6A (NP_002860.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	BT-474, OVCAR3, SW480, HT-29, 293T, SH-SY5Y, Mouse brain, Mouse lung, Mouse intestine

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human RAB6A (NP_002860.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSTG GDFG NPLR KFKL VFLG EQSV GKTS LITR FMYD SFDN TYQA TIGI DFLS KTMY LEDR TIRL QLWD TAGQ ERFR SLIP SYIR DSAA AVVV YDIT NVNS FQQT TKWI DDVR TERG SDVI IMLV GNKT DLAD KRQV SIEE GERK AKEL NVMF IETS AKAG YNVK QLFR RVAA ALPG MEST QDRS REDM IDIK LEKP QEQP
Gene ID:	5870
Uniprot:	P20340
Cellular Location:	Golgi apparatus, Golgi apparatus membrane, Lipid-anchor
Calculated MW:	11kDa/19kDa/23kDa
Observed MW:	33kDa

Synonyms:

RAB6A, RAB6

Background:

This gene encodes a member of the RAB family, which belongs to the small GTPase superfamily. GTPases of the RAB family bind to various effectors to regulate the targeting and fusion of transport carriers to acceptor compartments. This protein is located at the Golgi apparatus, which regulates trafficking in both a retrograde (from early endosomes and Golgi to the endoplasmic reticulum) and an anterograde (from the Golgi to the plasma membrane) directions. Myosin II is an effector of this protein in these processes. This protein is also involved in assembly of human cytomegalovirus (HCMV) by interacting with the cellular protein Bicaudal D1, which interacts with the HCMV virion tegument protein, pp150. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

UniProt Protein Function:	RAB6A: Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Has a low GTPase activity. Interacts with CCDC64; leads to its accumulation in the pericentrosomal region. Interacts with SCYL1BP1. Interacts with VSP52 and RABGAP1. Interacts with GCC2 (via its GRIP domain). Interacts with RAB6IP1 (via its RUN 1 domain). Isoform 1 interacts with RAB6KIFL. Isoform 2 does not interact with RAB6KIFL. Isoform 1 interacts with BICD1. Isoform 2 interacts with BICD1. Isoform 1 interacts with BICD2. Isoform 2 interacts with BICD2. Interacts with TMF1. Isoform 1 (GTP-bound) interacts with DYNLRB1; the interaction is direct. Isoform 2 (GDP-bound) interacts with DYNLRB1; the interaction is direct. Interacts with PIFO/C1orf88. Ubiquitous. Belongs to the small GTPase superfamily. Rab family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:G protein, monomeric, Rab; G protein, monomeric; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 11q13.3 Cellular Component: cytoplasmic vesicle; cytosol; Golgi apparatus; Golgi membrane; membrane; trans-Golgi network Molecular Function:GTP binding; GTPase activity; myosin V binding; protein binding; protein domain specific binding Biological Process: antigen processing and presentation; peptidyl-cysteine methylation; protein localization in Golgi apparatus; protein targeting to Golgi; retrograde vesicle-mediated transport, Golgi to ER; small GTPase mediated signal transduction; viral reproduction
NCBI Summary:	This gene encodes a member of the RAB family, which belongs to the small GTPase superfamily. GTPases of the RAB family bind to various effectors to regulate the targeting and fusion of transport carriers to acceptor compartments. This protein is located at the Golgi apparatus, which regulates trafficking in both a retrograde (from early endosomes and Golgi to the endoplasmic reticulum) and an anterograde (from the Golgi to the plasma membrane) directions. Myosin II is an effector of this protein in these processes. This protein is also involved in assembly of human cytomegalovirus (HCMV) by interacting with the cellular protein Bicaudal D1, which interacts with the HCMV virion tegument protein, pp150. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
UniProt Code:	P20340
NCBI GenInfo Identifier:	131796
NCBI Gene ID:	5870
NCBI Accession:	P20340.3
UniProt Secondary Accession:	P20340,Q1W5D8, Q5U0A8, Q9UBE4, A8K133, B7Z772, F5H668
UniProt Related Accession:	P20340
Molecular Weight:	19,935 Da
NCBI Full Name:	Ras-related protein Rab-6A
NCBI Synonym Full Names:	RAB6A, member RAS oncogene family
NCBI Official Symbol:	RAB6A
NCBI Official Synonym Symbols:	RAB6
NCBI Protein Information:	ras-related protein Rab-6A
UniProt Protein Name:	Ras-related protein Rab-6A
Protein Family:	Ras-related protein
UniProt Gene Name:	RAB6A
UniProt Entry Name:	RAB6A_HUMAN

	Western blot analysis of extracts of various cell lines, using RAB6A antibody (CAB5613) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
	Immunofluorescence analysis of HeLa cells using RAB6A antibody (CAB5613). Blue: DAPI for nuclear staining.

Antibodies

Anti-RAB6A Antibody (CAB12089)

RAB6A Antibody (PACO06978)

RAB6A Antibody (PACO11668)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)