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Anti-RNASEL Antibody (CAB9840)

SKU:
CAB9840
Product Type:
Antibody
Applications:
WB
IHC
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
  • Immunology Antibodies 3 Anti-RNASEL Antibody CAB9840
  • Immunology Antibodies 3 Anti-RNASEL Antibody CAB9840
  • Immunology Antibodies 3 Anti-RNASEL Antibody CAB9840
  • Immunology Antibodies 3 Anti-RNASEL Antibody CAB9840
€139 - €419
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Description

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Antibody Name:Anti-RNASEL Antibody
Antibody SKU:CAB9840
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 472-741 of human RNASEL (NP_066956.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HT-29, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 472-741 of human RNASEL (NP_066956.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VQEL HLSC GYTH QDLQ PQNI LIDS KKAA HLAD FDKS IKWA GDPQ EVKR DLED LGRL VLYV VKKG SISF EDLK AQSN EEVV QLSP DEET KDLI HRLF HPGE HVRD CLSD LLGH PFFW TWES RYRT LRNV GNES DIKT RKSE SEIL RLLQ PGPS EHSK SFDK WTTK INEC VMKK MNKF YEKR GNFY QNTV GDLL KFIR NLGE HIDE EKHK KMKL KIGD PSLY FQKT FPDL VIYV YTKL QNTE YRKH FPQT HSPN KPQC DGAG GASG LASP GC
Gene ID:6041
Uniprot:Q05823
Cellular Location:Cytoplasm, Mitochondrion
Calculated MW:73kDa/83kDa
Observed MW:84kDa
Synonyms:RNASEL, PRCA1, RNS4
Background:This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele.
UniProt Protein Function:Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. In the crosstalk between autophagy and apoptosis proposed to induce autophagy as an early stress response to small double-stranded RNA and at later stages of prolonged stress to activate caspase-dependent proteolytic cleavage of BECN1 to terminate autophagy and promote apoptosis (PubMed:26263979). Might play a central role in the regulation of mRNA turnover (PubMed:11585831). Cleaves 3' of UpNp dimers, with preference for UU and UA sequences, to sets of discrete products ranging from between 4 and 22 nucleotides in length.
NCBI Summary:This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele. [provided by RefSeq, Jul 2008]
UniProt Code:Q05823
NCBI GenInfo Identifier:10863929
NCBI Gene ID:6041
NCBI Accession:NP_066956.1
UniProt Secondary Accession:Q05823,Q5W0L2, Q6AI46,
UniProt Related Accession:Q05823
Molecular Weight:73,416 Da
NCBI Full Name:2-5A-dependent ribonuclease
NCBI Synonym Full Names:ribonuclease L
NCBI Official Symbol:RNASEL  
NCBI Official Synonym Symbols:RNS4; PRCA1  
NCBI Protein Information:2-5A-dependent ribonuclease
UniProt Protein Name:2-5A-dependent ribonuclease
UniProt Synonym Protein Names:Ribonuclease 4; Ribonuclease L; RNase L
Protein Family:2-5A-dependent ribonuclease
UniProt Gene Name:RNASEL  
Anti-RNASEL Polyclonal Antibody (CAB9840)Western blot analysis of extracts of various cell lines, using RNASEL antibody (CAB9840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-RNASEL Polyclonal Antibody (CAB9840)Immunohistochemistry of paraffin-embedded rat kidney using RNASEL antibody (CAB9840) at dilution of 1:100 (40x lens).
Anti-RNASEL Polyclonal Antibody (CAB9840)Immunohistochemistry of paraffin-embedded human lung cancer using RNASEL antibody (CAB9840) at dilution of 1:100 (40x lens).
Anti-RNASEL Polyclonal Antibody (CAB9840)Immunohistochemistry of paraffin-embedded mouse brain using RNASEL antibody (CAB9840) at dilution of 1:100 (40x lens).

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