Anti-RTN4 Antibody (CAB1752)

Antibody Name:	Anti-RTN4 Antibody
Antibody SKU:	CAB1752
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human RTN4.

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	mouse brain

Immunogen:	Recombinant protein of human RTN4.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	57142
Uniprot:	Q9NQC3
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:	22kDa/40kDa/42kDa/106kDa/108kDa/129kDa
Observed MW:	200kDa

Synonyms:

RTN4, ASY, NI220/250, NOGO, NSP, NSP-CL, Nbla00271, Nbla10545, RTN-X, RTN4-A, RTN4-B1, RTN4-B2, RTN4-C, NOGO-A, Nogo-B, Nogo-C, NOGOC

Background:

This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified.

UniProt Protein Function:	Nogo: Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Membrane protein, multi-pass; Cell development/differentiation; Motility/polarity/chemotaxis; Endoplasmic reticulum; Inhibitor Chromosomal Location of Human Ortholog: 2p16.3 Cellular Component: cell projection; cell soma; endoplasmic reticulum; plasma membrane; integral to endoplasmic reticulum membrane; nuclear envelope; intracellular Molecular Function:protein binding Biological Process: regulation of apoptosis; nerve growth factor receptor signaling pathway; cerebral cortex radial glia guided migration; apoptosis; axonal fasciculation; negative regulation of axonogenesis; negative regulation of axon extension; angiogenesis; negative regulation of cell growth; regulation of axonogenesis; regulation of cell migration
NCBI Summary:	This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NQC3
NCBI GenInfo Identifier:	17369290
NCBI Gene ID:	57142
NCBI Accession:	Q9NQC3.2
UniProt Secondary Accession:	Q9NQC3,O94962, Q7L7Q5, Q7L7Q6, Q7L7Q8, Q8IUA4, Q96B16 Q9BXG5, Q9H212, Q9H3I3, Q9UQ42, Q9Y293,
UniProt Related Accession:	Q9NQC3
Molecular Weight:	108,450 Da
NCBI Full Name:	Reticulon-4
NCBI Synonym Full Names:	reticulon 4
NCBI Official Symbol:	RTN4
NCBI Official Synonym Symbols:	ASY; NSP; NOGO; NOGOC; RTN-X; NOGO-A; NSP-CL; Nogo-B; Nogo-C; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; Nbla10545
NCBI Protein Information:	reticulon-4; foocen; Human NogoA; reticulon 5; My043 protein; neurite outgrowth inhibitor; neurite growth inhibitor 220; neuroendocrine-specific protein C homolog
UniProt Protein Name:	Reticulon-4
UniProt Synonym Protein Names:	Foocen; Neurite outgrowth inhibitor; Nogo protein; Neuroendocrine-specific protein; NSP; Neuroendocrine-specific protein C homolog; RTN-x; Reticulon-5
Protein Family:	Reticulon
UniProt Gene Name:	RTN4
UniProt Entry Name:	RTN4_HUMAN

	Western blot analysis of extracts of mouse brain, using RTN4 antibody (CAB1752) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5min.
	Immunofluorescence analysis of rat testis using RTN4 antibody (CAB1752) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of rat brain using RTN4 antibody (CAB1752) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse testis using RTN4 antibody (CAB1752) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse brain using RTN4 antibody (CAB1752) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-RTN4 Antibody (CAB3114)	Human Nogo / Reticulon 4 ELISA Kit
RTN4 Antibody (PACO01179)
RTN4 Antibody (PACO11982)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)