Anti-SETD2 Antibody (CAB3194)

Antibody Name:	Anti-SETD2 Antibody
Antibody SKU:	CAB3194
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 803-1103 of human SETD2 (NP_054878.5).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	NCI-H460, Mouse brain, Mouse kidney, Mouse liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 803-1103 of human SETD2 (NP_054878.5).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SLCN SEAE NIEP SVMK ISSN SFMN VHLE SKPV ICDS RNLT DHSK FACE EYKQ SIGS TSSA SVNH FDDL YQPI GSSG IASS LQSL PPGI KVDS LTLL KCGE NTSP VLDA VLKS KKSS EFLK HAGK ETIV EVGS DLPD SGKG FASR ENRR NNGL SGKC LQEA QEEG NSIL PERR GRPE ISLD ERGE GGHV HTSD DSEV VFSS CDLN LTME DSDG VTYA LKCD SSGH APEI VSTV HEDY SGSS ESSN DESD SEDT DSDD SSIP RNRL QSVV VVPK NSTL PMEE TSPC SSRS SQSY RHYS DHWE D
Gene ID:	29072
Uniprot:	Q9BYW2
Cellular Location:	Chromosome, Nucleus
Calculated MW:	175kDa/192kDa/287kDa
Observed MW:	300kDa

Synonyms:

SETD2, HBP231, HIF-1, HIP-1, HSPC069, HYPB, KMT3A, LLS, SET2, p231HBP

Background:

Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. This protein is a histone methyltransferase that is specific for lysine-36 of histone H3, and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II.

UniProt Protein Function:	SETD2: Histone methyltransferase that methylates 'Lys-36' of histone H3. H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation. Probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. Binds DNA at promoters. May also act as a transcription activator that binds to promoters. Binds to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression. Belongs to the histone-lysine methyltransferase family. SET2 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Amino Acid Metabolism - lysine degradation; Methyltransferase, protein lysine; EC 2.1.1.43; Methyltransferase Chromosomal Location of Human Ortholog: 3p21.31 Cellular Component: nucleoplasm; chromosome Molecular Function:protein binding; histone-lysine N-methyltransferase activity Biological Process: pericardium development; establishment and/or maintenance of chromatin architecture; stem cell development; mismatch repair; regulation of transcription, DNA-dependent; mesoderm morphogenesis; forebrain development; neural tube closure; RNA elongation from RNA polymerase II promoter; embryonic cranial skeleton morphogenesis; morphogenesis of a branching structure; angiogenesis
NCBI Summary:	Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. This protein is a histone methyltransferase that is specific for lysine-36 of histone H3, and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II. [provided by RefSeq, Aug 2008]
UniProt Code:	Q9BYW2
NCBI GenInfo Identifier:	296452963
NCBI Gene ID:	29072
NCBI Accession:	Q9BYW2.3
UniProt Secondary Accession:	Q9BYW2,O75397, O75405, Q17RW8, Q5BKS9, Q5QGN2, Q69YI5 Q6IN64, Q6ZN53, Q6ZS25, Q8N3R0, Q8TCN0,
UniProt Related Accession:	Q9BYW2
Molecular Weight:	2564
NCBI Full Name:	Histone-lysine N-methyltransferase SETD2
NCBI Synonym Full Names:	SET domain containing 2
NCBI Official Symbol:	SETD2
NCBI Official Synonym Symbols:	HYPB; SET2; HIF-1; HIP-1; KMT3A; HBP231; HSPC069; p231HBP
NCBI Protein Information:	histone-lysine N-methyltransferase SETD2; huntingtin yeast partner B; lysine N-methyltransferase 3A; huntingtin interacting protein 1; huntingtin-interacting protein B
UniProt Protein Name:	Histone-lysine N-methyltransferase SETD2
UniProt Synonym Protein Names:	HIF-1; Huntingtin yeast partner B; Huntingtin-interacting protein 1; HIP-1; Huntingtin-interacting protein B; Lysine N-methyltransferase 3A; SET domain-containing protein 2; hSET2; p231HBP
Protein Family:	Histone-lysine N-methyltransferase
UniProt Gene Name:	SETD2
UniProt Entry Name:	SETD2_HUMAN

	Western blot analysis of extracts of various cell lines, using SETD2 antibody (CAB3194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunofluorescence analysis of C6 cells using SETD2 Polyclonal Antibody (CAB3194) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using SETD2 Polyclonal Antibody (CAB3194) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-SETD2 Antibody (CAB11271)

Anti-SETD2 Antibody (CAB11757)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)