Anti-SMO Antibody (CAB3274)

Antibody Name:	Anti-SMO Antibody
Antibody SKU:	CAB3274
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 554-787 of human SMO (NP_005622.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 554-787 of human SMO (NP_005622.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GQSD DEPK RIKK SKMI AKAF SKRH ELLQ NPGQ ELSF SMHT VSHD GPVA GLAF DLNE PSAD VSSA WAQH VTKM VARR GAIL PQDI SVTP VATP VPPE EQAN LWLV EAEI SPEL QKRL GRKK KRRK RKKE VCPL APPP ELHP PAPA PSTI PRLP QLPR QKCL VAAG AWGA GDSC RQGA WTLV SNPF CPEP SPPQ DPFL PSAP APVA WAHG RRQG LGPI HSRT NLMD TELM DADS DF
Gene ID:	6608
Uniprot:	Q99835
Cellular Location:	Cell projection, Membrane, Multi-pass membrane protein, cilium
Calculated MW:	86kDa
Observed MW:	86kDa

Synonyms:	Gx, CRJS, SMOH, FZD11, SMO
Background:	The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.

UniProt Protein Function:	SMO: G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia. Belongs to the G-protein coupled receptor Fz/Smo family.
UniProt Protein Details:	Protein type:GPCR, Fz/Smo family; Receptor, GPCR; Membrane protein, multi-pass; Cell development/differentiation; Membrane protein, integral; Oncoprotein Chromosomal Location of Human Ortholog: 7q32.3 Cellular Component: caveola; Golgi apparatus; integral to membrane; intracellular membrane-bound organelle; plasma membrane Molecular Function:drug binding; G-protein coupled receptor activity; patched binding; protein binding; Wnt receptor activity; Wnt-protein binding Biological Process: activation of hh target transcription factor; forebrain morphogenesis; heart looping; midgut development; negative regulation of DNA binding; smoothened signaling pathway; ventral midline determination; Wnt receptor signaling pathway through beta-catenin
NCBI Summary:	The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex. [provided by RefSeq, Jul 2010]
UniProt Code:	Q99835
NCBI GenInfo Identifier:	6226142
NCBI Gene ID:	6608
NCBI Accession:	Q99835.1
UniProt Secondary Accession:	Q99835,A4D1K5,
UniProt Related Accession:	Q99835
Molecular Weight:	86,397 Da
NCBI Full Name:	Smoothened homolog
NCBI Synonym Full Names:	smoothened, frizzled class receptor
NCBI Official Symbol:	SMO
NCBI Official Synonym Symbols:	Gx; SMOH; FZD11
NCBI Protein Information:	smoothened homolog
UniProt Protein Name:	Smoothened homolog
UniProt Synonym Protein Names:	Protein Gx
Protein Family:	Smoothelin
UniProt Gene Name:	SMO
UniProt Entry Name:	SMO_HUMAN

	Western blot analysis of extracts of various cell lines, using SMO antibody (CAB3274) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat brain using SMO antibody (CAB3274) (40x lens).
	Immunofluorescence analysis of C6 cells using SMO Polyclonal Antibody (CAB3274) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using SMO Polyclonal Antibody (CAB3274) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-Phospho-SMO-S611 Antibody (CABP0936)

Anti-Phospho-SMO-S615 Antibody (CABP0937)

Anti-Phospho-SMO-S616 Antibody (CABP0938)

Anti-Phospho-SMO-S611/615 Antibody (CABP0939)

Anti-Phospho-SMO-S611/615/616 Antibody (CABP0940)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)