Anti-SPDEF Antibody (CAB14114)

Antibody Name:	Anti-SPDEF Antibody
Antibody SKU:	CAB14114
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human SPDEF (NP_036523.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Rat
Positive Samples:	Rat ovary

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human SPDEF (NP_036523.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGSA SPGL SSVS PSHL LLPP DTVS RTGL EKAA AGAV GLER RDWS PSPP ATPE QGLS AFYL SYFD MLYP EDSS WAAK APGA SSRE EPPE EPEQ CPVI DSQA PAGS LDLV PGGL TLEE HSLE QVQS MVVG EVLK DIET ACKL LNIT ADPM DWSP SNVQ KWLL WTEH QYRL PPMG KAFQ ELAG KELC AMSE EQFR QRSP LGGD VLHA HLDI WKSA AWMK ERTS PGAI HYCA STSE ESWT DSEV DSSC SGQP IHLW QFLK ELLL KPHS YGRF IRWL NKEK GIFK IEDS AQVA RLWG IRKN RPAM NYDK LSRS IRQY YKKG IIRK PDIS QRLV YQFV HPI
Gene ID:	25803
Uniprot:	O95238
Cellular Location:	Nucleus
Calculated MW:	35kDa/37kDa
Observed MW:	50kDa

Synonyms:

SPDEF, PDEF, bA375E1.3

Background:

The protein encoded by this gene belongs to the ETS family of transcription factors. It is highly expressed in the prostate epithelial cells, and functions as an androgen-independent transactivator of prostate-specific antigen (PSA) promoter. Higher expression of this protein has also been reported in brain, breast, lung and ovarian tumors, compared to the corresponding normal tissues, and it shows better tumor-association than other cancer-associated molecules, making it a more suitable target for developing specific cancer therapies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	SPDEF: May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT- 3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter. Belongs to the ETS family.
UniProt Protein Details:	Protein type:Transcription factor; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; DNA-binding Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: nucleus Molecular Function:sequence-specific DNA binding Biological Process: transcription from RNA polymerase II promoter; positive regulation of cell fate commitment; positive regulation of apoptosis; multicellular organismal development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; cell differentiation; negative regulation of cell fate commitment
NCBI Summary:	The protein encoded by this gene belongs to the ETS family of transcription factors. It is highly expressed in the prostate epithelial cells, and functions as an androgen-independent transactivator of prostate-specific antigen (PSA) promoter. Higher expression of this protein has also been reported in brain, breast, lung and ovarian tumors, compared to the corresponding normal tissues, and it shows better tumor-association than other cancer-associated molecules, making it a more suitable target for developing specific cancer therapies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
UniProt Code:	O95238
NCBI GenInfo Identifier:	74762123
NCBI Gene ID:	25803
NCBI Accession:	O95238.1
UniProt Secondary Accession:	O95238,B4DWH8, F5H778,
UniProt Related Accession:	O95238
Molecular Weight:	335
NCBI Full Name:	SAM pointed domain-containing Ets transcription factor
NCBI Synonym Full Names:	SAM pointed domain containing ETS transcription factor
NCBI Official Symbol:	SPDEF
NCBI Official Synonym Symbols:	PDEF; bA375E1.3
NCBI Protein Information:	SAM pointed domain-containing Ets transcription factor; prostate-specific Ets; prostate-derived Ets factor; prostate epithelium-specific Ets transcription factor
UniProt Protein Name:	SAM pointed domain-containing Ets transcription factor
UniProt Synonym Protein Names:	Prostate epithelium-specific Ets transcription factor; Prostate-specific Ets; Prostate-derived Ets factor
Protein Family:	SAM pointed domain-containing Ets transcription factor
UniProt Gene Name:	SPDEF
UniProt Entry Name:	SPDEF_HUMAN

	Western blot analysis of extracts of rat ovary, using SPDEF antibody (CAB14114) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human placenta using SPDEF antibody (CAB14114) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF7 cells using SPDEF antibody (CAB14114).

Antibodies

Anti-SPDEF Antibody (CAB6747)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)