Anti-SS18L1 Antibody (CAB8822)

Antibody Name:	Anti-SS18L1 Antibody
Antibody SKU:	CAB8822
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 90-270 of human SS18L1 (NP_945173.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HT-29, Raji, Mouse brain, Mouse liver, Rat brain, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 90-270 of human SS18L1 (NP_945173.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LTQS GSSQ GLHS QGSL SDAI STGL PPSS LLQG QIGN GPSH VSMQ QTAP NTLP TTSM SISG PGYS HAGP ASQG VPMQ GQGT IGNY VSRT NINM QSNP VSMM QQQA ATSH YSSA QGGS QHYQ GQSS IAMM GQGS QGSS MMGQ RPMA PYRP SQQG SSQQ YLGQ EEYY GEQY SHSQ GAAE PMGQ Q
Gene ID:	26039
Uniprot:	O75177
Cellular Location:	Chromosome, Nucleus, centromere, kinetochore
Calculated MW:	29kDa/33kDa/40kDa/42kDa/43kDa
Observed MW:	55kDa

Synonyms:	SS18L1, CREST, LP2261
Background:	This gene encodes a calcium-responsive transactivator which is an essential subunit of a neuron-specific chromatin-remodeling complex. The structure of this gene is similar to that of the SS18 gene. Mutations in this gene are involved in amyotrophic lateral sclerosis (ALS). Alternatively spliced transcript variants have been found for this gene.

UniProt Protein Function:	SS18L1: Transcriptional activator which is required for calcium- dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the SS18 family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 20q13.3 Cellular Component: kinetochore; nucleus Molecular Function:protein binding Biological Process: positive regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a calcium-responsive transactivator which is an essential subunit of a neuron-specific chromatin-remodeling complex. The structure of this gene is similar to that of the SS18 gene. Mutations in this gene are involved in amyotrophic lateral sclerosis (ALS). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014]
UniProt Code:	O75177
NCBI GenInfo Identifier:	20139981
NCBI Gene ID:	26039
NCBI Accession:	O75177.2
UniProt Secondary Accession:	O75177,Q5JXJ3, Q6MZV9, Q6NTH3, Q6XYD9, Q8NE69, Q9BR55 Q9H4K6, A6NNE3, A8K620, B3KWR8, E1P5H7,
UniProt Related Accession:	O75177
Molecular Weight:	43,408 Da
NCBI Full Name:	Calcium-responsive transactivator
NCBI Synonym Full Names:	SS18L1, nBAF chromatin remodeling complex subunit
NCBI Official Symbol:	SS18L1
NCBI Official Synonym Symbols:	CREST; LP2261
NCBI Protein Information:	calcium-responsive transactivator
UniProt Protein Name:	Calcium-responsive transactivator
UniProt Synonym Protein Names:	SS18-like protein 1; SYT homolog 1
Protein Family:	Calcium-responsive transactivator
UniProt Gene Name:	SS18L1
UniProt Entry Name:	CREST_HUMAN

	Western blot analysis of extracts of various cell lines, using SS18L1 antibody (CAB8822) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse brain using SS18L1 antibody (CAB8822) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using SS18L1 antibody (CAB8822) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using SS18L1 antibody (CAB8822) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)