Anti-STK26 Antibody (CAB16534)

Antibody Name:	Anti-STK26 Antibody
Antibody SKU:	CAB16534
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 297-416 of human STK26 (NP_057626.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HeLa, mouse thymus, mouse spleen, mouse lung, rat thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 297-416 of human STK26 (NP_057626.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EGHS DDES DSEG SDSE STSR ENNT HPEW SFTT VRKK PDPK KVQN GAEQ DLVQ TLSC LSMI ITPA FAEL KQQD ENNA SRNQ AIEE LEKS IAVA EAAC PGIT DKMV KKLI EKFQ KCSA DESP
Gene ID:	51765
Uniprot:	Q9P289
Cellular Location:	Cytoplasm, Cytoplasmic side, Golgi apparatus membrane, Peripheral membrane protein
Calculated MW:	37kDa/39kDa/46kDa
Observed MW:	47kDa

Synonyms:

STK26, MASK, MST4

Background:

The product of this gene is a member of the GCK group III family of kinases, which are a subset of the Ste20-like kinases. The encoded protein contains an amino-terminal kinase domain, and a carboxy-terminal regulatory domain that mediates homodimerization. The protein kinase localizes to the Golgi apparatus and is specifically activated by binding to the Golgi matrix protein GM130. It is also cleaved by caspase-3 in vitro, and may function in the apoptotic pathway. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

NCBI Summary:	The product of this gene is a member of the GCK group III family of kinases, which are a subset of the Ste20-like kinases. The encoded protein contains an amino-terminal kinase domain, and a carboxy-terminal regulatory domain that mediates homodimerization. The protein kinase localizes to the Golgi apparatus and is specifically activated by binding to the Golgi matrix protein GM130. It is also cleaved by caspase-3 in vitro, and may function in the apoptotic pathway. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9P289
NCBI GenInfo Identifier:	33878909
NCBI Gene ID:	51765
NCBI Accession:	BC017213
UniProt Related Accession:	Q9P289
Molecular Weight:
NCBI Full Name:	Homo sapiens serine/threonine protein kinase MST4, mRNA
NCBI Synonym Full Names:	serine/threonine kinase 26
NCBI Official Symbol:	STK26
NCBI Official Synonym Symbols:	MASK; MST4
NCBI Protein Information:	serine/threonine-protein kinase 26
UniProt Protein Name:
UniProt Gene Name:

	Western blot analysis of extracts of rat thymus, using STK26 antibody (CAB16534) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Western blot analysis of extracts of various cell lines, using STK26 antibody (CAB16534) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded Human thyroid cancer using STK26 Rabbit pAb (CAB16534) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Mouse spleen using STK26 Rabbit pAb (CAB16534) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)