Anti-STOM Antibody (CAB6372)

Antibody Name:	Anti-STOM Antibody
Antibody SKU:	CAB6372
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 55-288 of human STOM (NP_004090.4).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Mouse spleen, Mouse heart, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 55-288 of human STOM (NP_004090.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KIIK EYER AIIF RLGR ILQG GAKG PGLF FILP CTDS FIKV DMRT ISFD IPPQ EILT KDSV TISV DGVV YYRV QNAT LAVA NITN ADSA TRLL AQTT LRNV LGTK NLSQ ILSD REEI AHNM QSTL DDAT DAWG IKVE RVEI KDVK LPVQ LQRA MAAE AEAS REAR AKVI AAEG EMNA SRAL KEAS MVIT ESPA ALQL RYLQ TLTT IAAE KNST IVFP LPID MLQG IIGA KHSH LG
Gene ID:	2040
Uniprot:	P27105
Cellular Location:	Cell membrane, Cytoplasmic side, Cytoplasmic vesicle, Lipid-anchor, Melanosome, Peripheral membrane protein
Calculated MW:	13kDa/31kDa
Observed MW:	32kDa

Synonyms:

STOM, BND7, EPB7, EPB72, stomatin

Background:

This gene encodes a member of a highly conserved family of integral membrane proteins. The encoded protein localizes to the cell membrane of red blood cells and other cell types, where it may regulate ion channels and transporters. Loss of localization of the encoded protein is associated with hereditary stomatocytosis, a form of hemolytic anemia. There is a pseudogene for this gene on chromosome 6. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	stomatin: Thought to regulate cation conductance. May regulate ASIC2 and ASIC3 gating. Belongs to the band 7/mec-2 family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold; Membrane protein, integral Chromosomal Location of Human Ortholog: 9q34.1 Cellular Component: cytoplasm; cytoskeleton; endoplasmic reticulum; extracellular space; integral to plasma membrane; lipid raft; membrane; mitochondrion; vesicle Molecular Function:protein binding Biological Process: positive regulation of defense response to virus by host; protein homooligomerization Disease: Overhydrated Hereditary Stomatocytosis
NCBI Summary:	This gene encodes a member of a highly conserved family of integral membrane proteins. The encoded protein localizes to the cell membrane of red blood cells and other cell types, where it may regulate ion channels and transporters. Loss of localization of the encoded protein is associated with hereditary stomatocytosis, a form of hemolytic anemia. There is a pseudogene for this gene on chromosome 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]
UniProt Code:	P27105
NCBI GenInfo Identifier:	114823
NCBI Gene ID:	2040
NCBI Accession:	P27105.3
UniProt Secondary Accession:	P27105,Q14087, Q15609, Q5VX96, Q96FK4, B1AM77,
UniProt Related Accession:	P27105
Molecular Weight:	13,475 Da
NCBI Full Name:	Erythrocyte band 7 integral membrane protein
NCBI Synonym Full Names:	stomatin
NCBI Official Symbol:	STOM
NCBI Official Synonym Symbols:	BND7; EPB7; EPB72
NCBI Protein Information:	erythrocyte band 7 integral membrane protein
UniProt Protein Name:	Erythrocyte band 7 integral membrane protein
UniProt Synonym Protein Names:	Protein 7.2b; Stomatin
Protein Family:	Stomatin
UniProt Gene Name:	STOM
UniProt Entry Name:	STOM_HUMAN

	Western blot analysis of extracts of various cell lines, using STOM antibody (CAB6372) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using STOM antibody (CAB6372). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-STOM Antibody (CAB13975)	Human STOM (Stomatin) ELISA Kit (HUFI06285)
STOM Antibody (PACO12542)	Human Erythrocyte band 7 integral membrane protein (STOM) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)