Anti-SUGCT Antibody (CAB3499)

Antibody Name:	Anti-SUGCT Antibody
Antibody SKU:	CAB3499
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human SUGCT (NP_001180240.1).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, Mouse liver, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human SUGCT (NP_001180240.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPSE THAM LATL ARVA ALRR TCLF SGRG GGRG LWTG RPQS DMNN IKPL EGVK ILDL TRVL AGPF ATMN LGDL GAEV IKVE RPGA GDDT RTWG PPFV GTES TYYL SVNR NKKS IAVN IKDP KGVK IIKE LAAV CDVF VENY VPGK LSAM GLGY EDID EIAP HIIY CSIT GYGQ TGPI SQRA GYDA VASA VSGL MHIT GPEN GDPV RPGV AMTD LATG LYAY GAIM AGLI QKYK TGKG LFID CNLL SSQV AC
Gene ID:	79783
Uniprot:	Q9HAC7
Cellular Location:	Mitochondrion
Calculated MW:	43kDa/47kDa/48kDa/51kDa
Observed MW:	48kDa

Synonyms:	SUGCT, C7orf10, DERP13, GA3, ORF19
Background:	This gene encodes a protein that is similar to members of the CaiB/baiF CoA-transferase protein family. Mutations in this gene are associated with glutaric aciduria type III. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	SUGCT: Defects in C7orf10 are the cause of glutaric aciduria type 3 (GA3). GA3 is a metabolic disorder due to peroxisomal glutaryl-CoA oxidase deficiency and characterized by the excretion of abnormal quantities of glutaric acid but low 3- hydroxyglutaric acid. Belongs to the CaiB/BaiF CoA-transferase family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 2.8.3.13; Transferase Chromosomal Location of Human Ortholog: 7p14.1 Cellular Component: mitochondrion Molecular Function:succinate-hydroxymethylglutarate CoA-transferase activity Biological Process: metabolic process Disease: Glutaric Aciduria Iii
NCBI Summary:	This gene encodes a protein that is similar to members of the CaiB/baiF CoA-transferase protein family. Mutations in this gene are associated with glutaric aciduria type III. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]
UniProt Code:	Q9HAC7
NCBI GenInfo Identifier:	71152390
NCBI Gene ID:	79783
NCBI Accession:	Q9HAC7.2
UniProt Secondary Accession:	Q9HAC7,Q4KMW4, Q4KMW8, Q4KMZ0, Q8TE00, Q8TEY1, A4D1W5 B4DR73,
UniProt Related Accession:	Q9HAC7
Molecular Weight:	445
NCBI Full Name:	Succinate--hydroxymethylglutarate CoA-transferase
NCBI Synonym Full Names:	succinyl-CoA:glutarate-CoA transferase
NCBI Official Symbol:	SUGCT
NCBI Official Synonym Symbols:	ORF19; DERP13; C7orf10
NCBI Protein Information:	succinate--hydroxymethylglutarate CoA-transferase; Russel-Silver syndrome candidate; dermal papilla-derived protein 13; succinylCoA:glutarate-CoA transferase
UniProt Protein Name:	Succinate--hydroxymethylglutarate CoA-transferase
UniProt Synonym Protein Names:	Dermal papilla-derived protein 13; SuccinylCoA:glutarate-CoA transferase
Protein Family:	Succinate--hydroxymethylglutarate CoA-transferase
UniProt Gene Name:	SUGCT
UniProt Entry Name:	SUCHY_HUMAN

	Western blot analysis of extracts of various cell lines, using SUGCT antibody (CAB3499) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using SUGCT antibody (CAB3499) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

SUGCT Antibody (PACO08114)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)