Anti-TANK Antibody (CAB6763)

Antibody Name:	Anti-TANK Antibody
Antibody SKU:	CAB6763
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-119 of human TANK (NP_597841.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-119 of human TANK (NP_597841.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDKN IGEQ LNKA YEAF RQAC MDRD SAVK ELQQ KTEN YEQR IREQ QEQL SLQQ TIID KLKS QLLL VNST QDNN YGCV PLLE DSET RKNN LTLD QPQD KVIS GIAR EKLP KVDI ASAE SSI
Gene ID:	10010
Uniprot:	Q92844
Cellular Location:	Cytoplasm
Calculated MW:	13kDa/47kDa
Observed MW:	47kDa

Synonyms:

TANK, I-TRAF, ITRAF, TRAF2

Background:

The TRAF (tumor necrosis factor receptor-associated factor) family of proteins associate with and transduce signals from members of the tumor necrosis factor receptor superfamily. The protein encoded by this gene is found in the cytoplasm and can bind to TRAF1, TRAF2, or TRAF3, thereby inhibiting TRAF function by sequestering the TRAFs in a latent state in the cytoplasm. For example, the protein encoded by this gene can block TRAF2 binding to LMP1, the Epstein-Barr virus transforming protein, and inhibit LMP1-mediated NF-kappa-B activation. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	TANK: Acts as a regulator of TRAF function by maintaining them in a latent state. Overexpression inhibits TRAF2-mediated NF- kappa-B activation signaled by CD40, TNFR1 and TNFR2. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. May be involved in I-kappa-B-kinase (IKK) regulation; may function as an adapter for kinases such as TBK1 or IKBKE that can modulate IKK activity. Interacts with TBK1 (via TRAF-C domain). Interacts with TRAF1 (via TRAF-C domain). Interacts with TRAF2 (via TRAF-C domain); the interaction is disrupted by the phosphorylation of TANK by IKBKE. Interacts with TRAF3 (via TRAF-C domain); the interaction with TRAF3 is weaker than the interactions with TRAF1 and TRAF3. Interacts with IKBKG; the interaction is enhanced by IKBKE and TBK1. Part of a ternary complex consisting of TANK, IKBKB and IKBKG. Ubiquitous. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Adaptor/scaffold; C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 2q24.2 Cellular Component: cytoplasm; cytosol Molecular Function:metal ion binding; protein binding; ubiquitin protein ligase binding Biological Process: I-kappaB kinase/NF-kappaB cascade; innate immune response; MyD88-independent toll-like receptor signaling pathway; signal transduction; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor signaling pathway
NCBI Summary:	The TRAF (tumor necrosis factor receptor-associated factor) family of proteins associate with and transduce signals from members of the tumor necrosis factor receptor superfamily. The protein encoded by this gene is found in the cytoplasm and can bind to TRAF1, TRAF2, or TRAF3, thereby inhibiting TRAF function by sequestering the TRAFs in a latent state in the cytoplasm. For example, the protein encoded by this gene can block TRAF2 binding to LMP1, the Epstein-Barr virus transforming protein, and inhibit LMP1-mediated NF-kappa-B activation. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
UniProt Code:	Q92844
NCBI GenInfo Identifier:	143811466
NCBI Gene ID:	10010
NCBI Accession:	Q92844.2
UniProt Secondary Accession:	Q92844,Q7Z4J6, Q92885, D3DPB5,
UniProt Related Accession:	Q92844
Molecular Weight:	13,626 Da
NCBI Full Name:	TRAF family member-associated NF-kappa-B activator
NCBI Synonym Full Names:	TRAF family member associated NFKB activator
NCBI Official Symbol:	TANK
NCBI Official Synonym Symbols:	ITRAF; TRAF2; I-TRAF
NCBI Protein Information:	TRAF family member-associated NF-kappa-B activator
UniProt Protein Name:	TRAF family member-associated NF-kappa-B activator
UniProt Synonym Protein Names:	TRAF-interacting protein; I-TRAF
Protein Family:	Tankyrase
UniProt Gene Name:	TANK
UniProt Entry Name:	TANK_HUMAN

	Western blot analysis of extracts of HT-29 cells, using TANK antibody (CAB6763) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using TANK antibody (CAB6763). Blue: DAPI for nuclear staining.

Antibodies

Anti-TANK Antibody (CAB14501)

TANK Antibody (PACO02284)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)