Anti-TCHH Antibody (CAB15731)

Antibody Name:	Anti-TCHH Antibody
Antibody SKU:	CAB15731
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human TCHH (NP_009044.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, Jurakt, HeLa, NIH/3T3, Mouse brain, Mouse kidnay, Mouse lung, Mouse pancreas

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human TCHH (NP_009044.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RARC DGKE SLLQ DRRQ EEDQ RRFE PRDR QLEE EPGQ RRRQ KRQE QERE LAEG EEQS EKQE RLEQ RDRQ RRDE ELWR QRQE WQER EERR AEEE QLQS CKGH ETEE FPDE EQLR RREL LELR RKGR EEKQ QQRR ERQD RVFQ EEEE KEWR KRET VLRK EEEK LQEE EPQR QREL QEEE EQLR KLER QELR RERQ EEEQ QQQR L
Gene ID:	7062
Uniprot:	Q07283
Cellular Location:
Calculated MW:	253kDa
Observed MW:	280kDa

Synonyms:	TCHH, THH, THL, TRHY, UHS3
Background:	The protein encoded by this gene forms crosslinked complexes with itself and keratin intermediate filaments to provide mechanical strength to the hair follicle inner root sheath. The encoded protein also is important for structural integrity of the filiform papillae of the tongue. Defects in this gene are a cause of uncombable hair syndrome.

UniProt Protein Function:	Intermediate filament-associated protein that associates in regular arrays with keratin intermediate filaments (KIF) of the inner root sheath cells of the hair follicle and the granular layer of the epidermis. It later becomes cross-linked to KIF by isodipeptide bonds. It may serve as scaffold protein, together with involucrin, in the organization of the cell envelope or even anchor the cell envelope to the KIF network. It may be involved in its own calcium-dependent postsynthetic processing during terminal differentiation.
NCBI Summary:	Trichohyalin confers mechanical strength to the hair follicle inner root sheath and to other toughened epithelial tissues, such as the hard palate and filiform ridges of the tongue, by forming multiple complex crosslinks with itself and with other structural proteins (Steinert et al., 2003 [PubMed 12853460]).[supplied by OMIM, Dec 2009]
UniProt Code:	Q07283
NCBI GenInfo Identifier:	148746195
NCBI Gene ID:	7062
NCBI Accession:	NP_009044.2
UniProt Secondary Accession:	Q07283,Q5VUI3,
UniProt Related Accession:	Q07283
Molecular Weight:	253,925 Da
NCBI Full Name:	trichohyalin
NCBI Synonym Full Names:	trichohyalin
NCBI Official Symbol:	TCHH
NCBI Official Synonym Symbols:	THH; THL; TRHY; UHS3
NCBI Protein Information:	trichohyalin
UniProt Protein Name:	Trichohyalin
Protein Family:	Trichohyalin
UniProt Gene Name:	TCHH

Western blot analysis of extracts of various cell lines, using TCHH antibody (CAB15731) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)