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Anti-TNFSF12 Antibody (CAB16458)

SKU:
CAB16458
Product Type:
Antibody
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cardiovascular
€139 - €419
Frequently bought together:

Description

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Antibody Name:Anti-TNFSF12 Antibody
Antibody SKU:CAB16458
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human TNFSF12
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Mouse, Rat
Positive Samples:Rat kidney
Immunogen:Recombinant protein of human TNFSF12
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:8742
Uniprot:O43508
Cellular Location:Cell membrane, Secreted, Single-pass membrane protein, Single-pass type II membrane protein
Calculated MW:27kDa/36kDa
Observed MW:27kDa
Synonyms:TNFSF12, APO3L, DR3LG, TNLG4A, TWEAK
Background:The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine, which exists in both membrane-bound and secreted forms, can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Alternative splicing results in multiple transcript variants. Some transcripts skip the last exon of this gene and continue into the second exon of the neighboring TNFSF13 gene; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13.
UniProt Protein Function:TNFSF12: Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Homotrimer (Potential). Interacts with the angiogenic factor AGGF1/VG5Q. Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain. Belongs to the tumor necrosis factor family.
UniProt Protein Details:

Protein type:Apoptosis; Membrane protein, integral; Motility/polarity/chemotaxis; Cytokine

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: extracellular space; perinuclear region of cytoplasm; integral to plasma membrane

Molecular Function:protein binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: positive regulation of angiogenesis; apoptosis; positive regulation of protein catabolic process; endothelial cell migration; immune response; positive regulation of endothelial cell proliferation; angiogenesis; cell differentiation; signal transduction

NCBI Summary:This gene encodes a member of the tumor necrosis factor superfamily. It encodes a hybrid protein composed of the cytoplasmic and transmembrane domains of family member 12 fused to the C-terminal domain of family member 13. The hybrid protein is membrane anchored and presents the receptor-binding domain of family member 13 at the cell surface. It stimulates cycling in T- and B-lymphoma cell lines. [provided by RefSeq, Jul 2008]
UniProt Code:O43508
NCBI GenInfo Identifier:21362987
NCBI Gene ID:407977
NCBI Accession:O43508.1
UniProt Secondary Accession:O43508,Q8IZK7, Q8WUZ7,
Molecular Weight:36,589 Da
NCBI Full Name:Tumor necrosis factor ligand superfamily member 12
NCBI Synonym Full Names:TNFSF12-TNFSF13 readthrough
NCBI Official Symbol:TNFSF12-TNFSF13  
NCBI Official Synonym Symbols:TWE-PRIL  
NCBI Protein Information:TNFSF12-TNFSF13 protein; tumor necrosis factor (ligand) superfamily, member 12-member 13
UniProt Protein Name:Tumor necrosis factor ligand superfamily member 12
UniProt Synonym Protein Names:APO3 ligand; TNF-related weak inducer of apoptosis; TWEAK
UniProt Gene Name:TNFSF12  
UniProt Entry Name:TNF12_HUMAN
Anti-TNFSF12 Antibody (CAB16458)Western blot analysis of extracts of rat kidney, using TNFSF12 antibody (CAB16458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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