Anti-TONSL Antibody (CAB13035)

Antibody Name:	Anti-TONSL Antibody
Antibody SKU:	CAB13035
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 160-390 of human TONSL (NP_038460.4).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Rat spleen, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 160-390 of human TONSL (NP_038460.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MRTR LYLN LGLT FESL QQTA LCND YFRK SIFL AEQN HLYE DLFR ARYN LGTI HWRA GQHS QAMR CLEG AREC AHTM RKRF MESE CCVV IAQV LQDL GDFL AAKR ALKK AYRL GSQK PVQR AAIC QNLQ HVLA VVRL QQQL EEAE GRDP QGAM VICE QLGD LFSK AGDF PRAA EAYQ KQLR FAEL LDRP GAER AIIH VSLA TTLG DMKD HHGA VRHY EEEL RLRS GNVL EEA
Gene ID:	4796
Uniprot:	Q96HA7
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	150kDa
Observed MW:	151kDa

Synonyms:

TONSL, IKBR, NFKBIL2

Background:

The protein encoded by this gene is thought to be a negative regulator of NF-kappa-B mediated transcription. The encoded protein may bind NF-kappa-B complexes and trap them in the cytoplasm, preventing them from entering the nucleus and interacting with the DNA. Phosphorylation of this protein targets it for degradation by the ubiquitination pathway, which frees the NF-kappa-B complexes to enter the nucleus.

UniProt Protein Function:	NFKBIL2: Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork- associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, may act as a scaffold. Belongs to the Tonsoku family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: DNA replication factor A complex; MCM complex; nuclear replication fork; nucleoplasm Molecular Function:histone binding; protein binding; transcription corepressor activity Biological Process: cytoplasmic sequestering of transcription factor; double-strand break repair via homologous recombination; replication fork processing
NCBI Summary:	The protein encoded by this gene is thought to be a negative regulator of NF-kappa-B mediated transcription. The encoded protein may bind NF-kappa-B complexes and trap them in the cytoplasm, preventing them from entering the nucleus and interacting with the DNA. Phosphorylation of this protein targets it for degradation by the ubiquitination pathway, which frees the NF-kappa-B complexes to enter the nucleus. [provided by RefSeq, Jul 2008]
UniProt Code:	Q96HA7
NCBI GenInfo Identifier:	182662416
NCBI Gene ID:	4796
NCBI Accession:	Q96HA7.2
UniProt Secondary Accession:	Q96HA7,Q13006, Q9UGJ2, B5MDP0, C9JKB1, C9JNV8,
UniProt Related Accession:	Q96HA7
Molecular Weight:	150,894 Da
NCBI Full Name:	Tonsoku-like protein
NCBI Synonym Full Names:	tonsoku-like, DNA repair protein
NCBI Official Symbol:	TONSL
NCBI Official Synonym Symbols:	IKBR; NFKBIL2
NCBI Protein Information:	tonsoku-like protein
UniProt Protein Name:	Tonsoku-like protein
UniProt Synonym Protein Names:	Inhibitor of kappa B-related protein; I-kappa-B-related protein; IkappaBR; NF-kappa-B inhibitor-like protein 2; Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2
UniProt Gene Name:	TONSL
UniProt Entry Name:	TONSL_HUMAN

	Western blot analysis of extracts of various cell lines, using TONSL antibody (CAB13035) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts of mouse brain, using TONSL antibody (CAB13035) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)