Anti-TRIM27 Antibody (CAB6405)

Antibody Name:	Anti-TRIM27 Antibody
Antibody SKU:	CAB6405
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 214-513 of human TRIM27 (NP_006501.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	Jurkat, mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 214-513 of human TRIM27 (NP_006501.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SING AITQ FSCN ISHL SSLI AQLE EKQQ QPTR ELLQ DIGD TLSR AERI RIPE PWIT PPDL QEKI HIFA QKCL FLTE SLKQ FTEK MQSD MEKI QELR EAQL YSVD VTLD PDTA YPSL ILSD NLRQ VRYS YLQQ DLPD NPER FNLF PCVL GSPC FIAG RHYW EVEV GDKA KWTI GVCE DSVC RKGG VTSA PQNG FWAV SLWY GKEY WALT SPMT ALPL RTPL QRVG IFLD YDAG EVSF YNVT ERCH TFTF SHAT FCGP VRPY FSLS YSGG KSAA PLII CPMS GIDG FSGH VGNH GHSM ETSP
Gene ID:	5987
Uniprot:	P14373
Cellular Location:	Cytoplasm, Early endosome, Nucleus, PML body
Calculated MW:	41kDa/58kDa
Observed MW:	59kDa

Synonyms:

TRIM27, RFP, RNF76

Background:

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein.

UniProt Protein Function:	TRIM27: Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA. E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Defects in TRIM27 are a cause of thyroid papillary carcinoma (TPC). TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. A chromosomal aberration involving TRIM27/RFP is found in thyroid papillary carcinomas. Translocation t(6;10)(p21.3;q11.2) with RET. The translocation generates TRIM27/RET and delta TRIM27/RET oncogenes. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; EC 6.3.2.-; Oncoprotein; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 6p22 Cellular Component: cytoplasm; endosome; integral to plasma membrane; membrane; nuclear membrane; nucleoplasm; nucleus; retromer complex Molecular Function:metal ion binding; nucleic acid binding; protein binding; transmembrane receptor protein tyrosine kinase activity; ubiquitin-protein ligase activity Biological Process: cell proliferation; innate immune response; negative regulation of adaptive immune response; negative regulation of gene expression, epigenetic; negative regulation of protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; negative regulation of viral transcription; positive regulation of transcription factor activity; retrograde transport, endosome to Golgi; spermatogenesis
NCBI Summary:	This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq, Jul 2008]
UniProt Code:	P14373
NCBI GenInfo Identifier:	132517
NCBI Gene ID:	5987
NCBI Accession:	P14373.1
UniProt Secondary Accession:	P14373,Q5RJA8, Q5ST26, Q6LA73, Q6NXR9, Q9BZY6, Q9UJL3 A2BE15,
UniProt Related Accession:	P14373
Molecular Weight:	41,439 Da
NCBI Full Name:	Zinc finger protein RFP
NCBI Synonym Full Names:	tripartite motif containing 27
NCBI Official Symbol:	TRIM27
NCBI Official Synonym Symbols:	RFP; RNF76
NCBI Protein Information:	zinc finger protein RFP
UniProt Protein Name:	Zinc finger protein RFP
UniProt Synonym Protein Names:	RING finger protein 76; Ret finger protein; Tripartite motif-containing protein 27
Protein Family:	Zinc finger protein
UniProt Gene Name:	TRIM27
UniProt Entry Name:	TRI27_HUMAN

	Western blot analysis of extracts of various cell lines, using TRIM27 antibody (CAB6405) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human esophagus using TRIM27 Antibody (CAB6405) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse stomach using TRIM27 Antibody (CAB6405) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using TRIM27 antibody (CAB6405). Blue: DAPI for nuclear staining.

Antibodies

Anti-TRIM27 Antibody (CAB16065)

TRIM27 Antibody (PACO12911)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)