Anti-TXK Antibody (CAB14792)

Antibody Name:	Anti-TXK Antibody
Antibody SKU:	CAB14792
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human TXK (NP_003319.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Rat
Positive Samples:	Rat thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human TXK (NP_003319.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MILS SYNT IQSV FCCC CCCS VQKR QMRT QISL STDE ELPE KYTQ RRRP WLSQ LSNK KQSN TGRV QPSK RKPL PPLP PSEV AEEK IQVK ALYD FLPR EPCN LALR RAEE YLIL EKYN PHWW KARD RLGN EGLI PSNY VTEN KITN LEIY EWYH RNIT RNQA EHLL RQES KEGA FIVR DSRH
Gene ID:	7294
Uniprot:	P42681
Cellular Location:	Cell membrane, Cytoplasm, Nucleus, Peripheral membrane protein
Calculated MW:	61kDa
Observed MW:	85kDa

Synonyms:	TXK, BTKL, PSCTK5, PTK4, RLK, TKL
Background:

UniProt Protein Function:	TXK: Non-receptor tyrosine kinase that plays a redundant role with ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T- cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of TXK to the cell membrane, where it is phosphorylated at Tyr-420. Phosphorylation leads to TXK full activation. Contributes also to signaling from many receptors and participates in multiple downstream pathways, including regulation of the actin cytoskekleton. Like ITK, can phosphorylate PLCG1, leading to its localization in lipid rafts and activation, followed by subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. With PARP1 and EEF1A1, TXK forms a complex that acts as a T-helper 1 (Th1) cell- specific transcription factor and binds the promoter of IFNG to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Phosphorylates both PARP1 and EEF1A1. Phosphorylates also key sites in LCP2 leading to the up-regulation of Th1 preferred cytokine IL-2. Phosphorylates 'Tyr- 201' of CTLA4 which leads to the association of PI-3 kinase with the CTLA4 receptor. Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
UniProt Protein Details:	Protein type:Protein kinase, TK; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Kinase, protein; TK group; Tec family Chromosomal Location of Human Ortholog: 4p12 Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm; nucleus Molecular Function:protein binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding Biological Process: adaptive immune response; transcription, DNA-dependent; protein amino acid autophosphorylation; cytokine production; T cell receptor signaling pathway; protein amino acid phosphorylation; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; interleukin-4 production; B cell receptor signaling pathway; positive regulation of interferon-gamma production; phospholipase C activation; interferon-gamma production; positive regulation of transcription from RNA polymerase II promoter; NK T cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation
UniProt Code:	P42681
NCBI GenInfo Identifier:	148596974
NCBI Gene ID:	7294
NCBI Accession:	NP_003319.2
UniProt Secondary Accession:	P42681,Q14220,
UniProt Related Accession:	P42681
Molecular Weight:	61,258 Da
NCBI Full Name:	tyrosine-protein kinase TXK
NCBI Synonym Full Names:	TXK tyrosine kinase
NCBI Official Symbol:	TXK
NCBI Official Synonym Symbols:	RLK; TKL; BTKL; PTK4; PSCTK5
NCBI Protein Information:	tyrosine-protein kinase TXK; tyrosine kinase; protein-tyrosine kinase 4; resting lymphocyte kinase; PTK4 protein tyrosine kinase 4
UniProt Protein Name:	Tyrosine-protein kinase TXK
UniProt Synonym Protein Names:	Protein-tyrosine kinase 4; Resting lymphocyte kinase
Protein Family:	Tyrosine-protein kinase
UniProt Gene Name:	TXK
UniProt Entry Name:	TXK_HUMAN

Western blot analysis of extracts of rat thymus, using TXK antibody (CAB14792) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s.

Antibodies

TXK Antibody (PACO05272)

TXK Antibody (PACO12996)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)