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Anti-UBR4 Antibody (CAB12193)

SKU:
CAB12193
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
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Description

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Antibody Name:Anti-UBR4 Antibody
Antibody SKU:CAB12193
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 4884-5183 of human UBR4 (NP_065816.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 4884-5183 of human UBR4 (NP_065816.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NIVH YDCH LAAV RLAR GREE WESA ALQN ANTK CNGL LPVW GPHV PESA FATC LARH NTYL QECT GQRE PTYQ LNIH DIKL LFLR FAME QSFS ADTG GGGR ESNI HLIP YIIH TVLY VLNT TRAT SREE KNLQ GFLE QPKE KWVE SAFE VDGP YYFT VLAL HILP PEQW RATR VEIL RRLL VTSQ ARAV APGG ATRL TDKA VKDY SAYR SSLL FWAL VDLI YNMF KKVP TSNT EGGW SCSL AEYI RHND MPIY EAAD KALK TFQE EFMP VETF SEFL DVAG LLSE ITDP ESFL KDLL NSVP
Gene ID:23352
Uniprot:Q5T4S7
Cellular Location:Cytoplasm, Membrane, Multi-pass membrane protein, Nucleus, cytoskeleton
Calculated MW:23kDa/270kDa/571kDa/573kDa/575kDa
Observed MW:600kDa
Synonyms:UBR4, RBAF600, ZUBR1, p600
Background:The protein encoded by this gene is an E3 ubiquitin-protein ligase that interacts with the retinoblastoma-associated protein in the nucleus and with calcium-bound calmodulin in the cytoplasm. The encoded protein appears to be a cytoskeletal component in the cytoplasm and part of the chromatin scaffold in the nucleus. In addition, this protein is a target of the human papillomavirus type 16 E7 oncoprotein.
UniProt Protein Function:UBR4: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Regulates integrin-mediated signaling. May play a role in activation of FAK in response to cell-matrix interactions. Belongs to the UBR4 family. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 6.3.2.-; EC 6.3.2.19; Ligase; Membrane protein, integral; Membrane protein, multi-pass; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: centrosome; cytosol; membrane; nucleoplasm; plasma membrane

Molecular Function:protein binding; ubiquitin-protein ligase activity

Biological Process: neutrophil degranulation; protein ubiquitination during ubiquitin-dependent protein catabolic process

NCBI Summary:The protein encoded by this gene is an E3 ubiquitin-protein ligase that interacts with the retinoblastoma-associated protein in the nucleus and with calcium-bound calmodulin in the cytoplasm. The encoded protein appears to be a cytoskeletal component in the cytoplasm and part of the chromatin scaffold in the nucleus. In addition, this protein is a target of the human papillomavirus type 16 E7 oncoprotein. [provided by RefSeq, Aug 2010]
UniProt Code:Q5T4S7
NCBI GenInfo Identifier:74744979
NCBI Gene ID:23352
NCBI Accession:Q5T4S7.1
UniProt Secondary Accession:Q5T4S7,O60646, O75050, Q4QRK5, Q5T4S8, Q5T4S9, Q5TBN8 Q5TBP2, Q6DKH8, A8MPT2, A8MQ33, A8MQB1,
UniProt Related Accession:Q5T4S7
Molecular Weight:574kDa
NCBI Full Name:E3 ubiquitin-protein ligase UBR4
NCBI Synonym Full Names:ubiquitin protein ligase E3 component n-recognin 4
NCBI Official Symbol:UBR4  
NCBI Official Synonym Symbols:p600; ZUBR1; RBAF600  
NCBI Protein Information:E3 ubiquitin-protein ligase UBR4
UniProt Protein Name:E3 ubiquitin-protein ligase UBR4
UniProt Synonym Protein Names:600 kDa retinoblastoma protein-associated factor; N-recognin-4; RING-type E3 ubiquitin transferase UBR4; Retinoblastoma-associated factor of 600 kDa; RBAF600; p600; Zinc finger UBR1-type protein 1
Protein Family:E3 ubiquitin-protein ligase
UniProt Gene Name:UBR4  
Anti-UBR4 Antibody (CAB12193)Western blot analysis of extracts of HeLa cells, using UBR4 antibody (CAB12193) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

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