Anti-WBSCR22 Antibody (CAB7317)

Antibody Name:	Anti-WBSCR22 Antibody
Antibody SKU:	CAB7317
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human WBSCR22 (NP_059998.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Rat
Positive Samples:	NCI-H460, A-549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human WBSCR22 (NP_059998.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASR GRRP EHGG PPEL FYDE TEAR KYVR NSRM IDIQ TRMA GRAL ELLY LPEN KPCY LLDI GCGT GLSG SYLS DEGH YWVG LDIS PAML DEAV DREI EGDL LLGD MGQG IPFK PGTF DGCI SISA VQWL CNAN KKSE NPAK RLYC FFAS LFSV LVRG SRAV LQLY PENS EQLE LITT QATK AGFS GGMV VDYP NSAK AKKF YLCL FSGP STFI PEGL SENQ DEVE PRES VFTN ERFP LRMS RRGM VRKS RAWV LEKK ERHR RQGR EVRP DTQY TGRK RKPR F
Gene ID:	114049
Uniprot:	O43709
Cellular Location:	Cytoplasm, Nucleus, nucleolus
Calculated MW:	24kDa/31kDa/33kDa
Observed MW:	36kDa

Synonyms:

BUD23, HASJ4442, HUSSY-3, MERM1, PP3381, WBMT, WBSCR22

Background:

This gene encodes a protein containing a nuclear localization signal and an S-adenosyl-L-methionine binding motif typical of methyltransferases, suggesting that the encoded protein may act on DNA methylation. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternatively spliced transcript variants have been found.

UniProt Protein Function:	WBSCR22: Methyltransferase that may act on DNA. WBSCR22 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of WBSCR22 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. Belongs to the methyltransferase superfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Other Amino Acids Metabolism - selenoamino acid; EC 2.1.1.-; Amino Acid Metabolism - tyrosine; Methyltransferase; Amino Acid Metabolism - histidine; Lipid Metabolism - androgen and estrogen Chromosomal Location of Human Ortholog: 7q11.23 Cellular Component: cytoplasm; nucleolus Molecular Function:methyltransferase activity Biological Process: methylation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; chromatin modification; rRNA processing
NCBI Summary:	This gene encodes a protein containing a nuclear localization signal and an S-adenosyl-L-methionine binding motif typical of methyltransferases, suggesting that the encoded protein may act on DNA methylation. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternatively spliced transcript variants have been found. [provided by RefSeq, Feb 2011]
UniProt Code:	O43709
NCBI GenInfo Identifier:	23831505
NCBI Gene ID:	114049
NCBI Accession:	O43709.2
UniProt Secondary Accession:	O43709,Q96P12, Q9BQ58, Q9HBP9, A8K501, C9K060,
UniProt Related Accession:	O43709
Molecular Weight:	281
NCBI Full Name:	Probable 18S rRNA (guanine-N(7))-methyltransferase
NCBI Synonym Full Names:	Williams Beuren syndrome chromosome region 22
NCBI Official Symbol:	WBSCR22
NCBI Official Synonym Symbols:	WBMT; MERM1; PP3381; HUSSY-3; HASJ4442
NCBI Protein Information:	probable 18S rRNA (guanine-N(7))-methyltransferase; probable 18S rRNA (guanine-N(7))-methyltransferase; bud site selection protein 23 homolog; metastasis-related methyltransferase 1; ribosome biogenesis methyltransferase WBSCR22; Williams-Beuren syndrome chromosomal region 22 protein; Williams-Beuren candidate region putative methyltransferase
UniProt Protein Name:	Probable 18S rRNA (guanine-N(7))-methyltransferase
UniProt Synonym Protein Names:	Bud site selection protein 23 homolog; Metastasis-related methyltransferase 1; Williams-Beuren syndrome chromosomal region 22 protein
Protein Family:	Probable 18S rRNA
UniProt Gene Name:	WBSCR22
UniProt Entry Name:	WBS22_HUMAN

	Western blot analysis of extracts of various cell lines, using WBSCR22 antibody (CAB7317) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat spleen using WBSCR22 antibody (CAB7317) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney using WBSCR22 antibody (CAB7317) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A-549 cells using WBSCR22 antibody (CAB7317).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)